Abstract: Cistanche tubulosa is endemic to Xinjiang.In order to make full use of its resources,on the basis of single factor tests,the ultrasonic microwave extraction technology of phenylethanoid glycosides from Cistanche tubulosa was studied through the response surface optimization method.And the characteristics was analyzed by the fourier infrared spectrum.The results showed that the ultrasonic frequency 40 k Hz,the ultrasonic power was 50 W,the extraction time was 15 min. The optimum extraction conditions by quadratic regression model were as follows extraction concentration 50%,ratio of liquid- solid 14 ∶ 1( m L·g^-1),extraction microwave power 100 W. The theoretical value of phenylethanoid glycosides yield was 49.87%,under this condition the verification value was49.2%,which was closed to the theoretical value.Infrared spectrum analysis results showed that ultrasonic microwave extraction was Cistanche tubulosa phenylethanoid glycosides compounds.