Optimization of extraction and physicochemical property of polysaccharides from lotus root
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摘要: 采用响应面法优化莲藕多糖的提取工艺,并初步探析多糖的理化特性。在单因素实验基础上,选取提取温度、提取时间和均质转速为影响因素,以莲藕多糖提取得率为响应值建立三因素三水平回归模型。基于回归模型分析和验证实验确定莲藕多糖提取的最佳工艺条件为:提取温度89℃、提取时间121 min、均质转速11800 r/min,该条件下的多糖得率预测值为5.64%,实际值为5.88%。莲藕粗多糖经Sevage法脱蛋白后,DPPH自由基清除能力明显增强,其分子量小于24.2 ku且主要由吡喃型单糖组成。Abstract: Response surface methodology( RSM) was applied to optimize the extraction conditions of water- soluble polysaccharides from lotus root. The physicochemical properties of polysaccharides were then analyzed. Three independent variables,extraction temperature,extraction time and shearing speed,were chosen to establish the quadric regression equation for predicting the yield of polysaccharides. The optimum extraction conditions were obtained as follows: extraction temperature 89 ℃,extraction time 121 min and shear speed rate 11800 r / min,which led to estimated and observed values of maximal yield of polysaccharides of 5.64% and 5.88%,respectively.Crude polysaccharides extracted from lotus root were purified by Sevage method.The purified polysaccharides showed a stronger DPPH radical scavenging activity compared with the crude ones. Their molecular weights were less than24.2 ku and mainly composed of pyranoid monosaccharides.
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