Purification of anthocyanins from purple sweet potato by combination of ultrafiltration and macroporous resin
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摘要: 利用超滤和大孔树脂联用的方法对紫薯花色苷粗提液进行了纯化,考查了不同截留分子量超滤膜对花色苷粗提液的纯化效果。结果表明:截留分子量为10 ku的膜可有效透过花色苷和截留大分子物质,花色苷的透过率为86.54%,蛋白质截留率为93.67%,透过液的可溶性固形物含量降低了14.29%,透光率提高了23.78%。通过对6种不同极性的大孔吸附树脂对超滤透过液中花色苷的吸附及解吸能力的考查,发现LS-305的吸附性能较好。其动态吸附及解吸条件为:上样流速5.2 BV/h,上样量35 BV;60%乙醇洗脱体积为9 BV可洗脱完全。花色苷产品色价由纯化前的1.59提高到纯化后的32.89。HPLC分析表明超滤和树脂联用纯化方法未对紫薯花色苷成分造成影响。Abstract: The anthocyanins extract from purple sweet potato was purified using ultrafiltration and macroporous resin. The effect of different molecular weight cutoff( MWCO) ultrafiltration membranes to remove the macromolecular substances was investigated. The results showed that the 10 ku membrane could effectively remove the macromolecular substances in the extract. The protein entrapment rate was 93.67%,and the anthocyanins through rate was 86.54%.The soluble solids content was reduced by 14.29%,and light transmittance increased by 23.78%.The adsorption and desorption ability for anthocyanins of six kind of macroporous resins were evaluated.It turned out that LS- 305 was the most suitable resin for anthocyanins. The dynamic adsorption and desorption conditions as follows: flow rate of 5.2 bed volume( BV) / h,the sample volume of 35 BV,60% ethanol used to elute the anthocyanins,and volume of 9 BV could elute anthocyanins completely.As a result,the color value of the anthocyanins from purple sweet potato increased from 1.59 to 32.89 after purification.HPLC analysis showed that ultrafiltration and macroporous resin did not affect the anthocyanins component.
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Keywords:
- purple sweet potato /
- anthocyanins /
- ultrafiltration /
- macroporous resin /
- purification
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[1] Wu Y,Wang Y,Zhang W,et al.Extraction and preliminary purification of anthocyanins from grape juice in aqueous twophase system[J].Separation and Purification Technology,2014,124:170-178.
[2] Giusti MM,Wrolstad RE.Acylatedanthocyanins from edible sources and their applicationsin food systems[J].Biochemical Engineering Journal,2003,14(3):217-225.
[3] 鲍诚,李玲,汤海宾,等.紫甘薯花色苷酶法提取及纯化[J].食品科学,2012,33(16):59-62. [4] 施鹏飞,肖海峻,罗红霞,等.响应面分析法优化紫薯花青素提取工艺[J].食品工业科技,2014,35(20):322-326. [5] Castaeda-Ovando A,Pacheco-Hernández Md L,PáezHernández ME,et al.Chemical studies of anthocyanins:A review[J].Food Chemistry,2009,113(4):859-871.
[6] 刘洁,叶婷婷.酶-微波辅助提取紫薯花青素的工艺研究[J].广州化工,2012,40(21):60-62. [7] 王宏兹,李进伟,范柳萍.超声-微波协同萃取法提取紫薯色素[J].安徽农业科学,2010,38(33):18664-18666. [8] 季秀玲.紫薯花色苷纯化工艺研究[D].南昌:江西农业大学,2014. [9] 万莹,杨青,谢鑫,等.膜技术用于紫薯花色苷色素分离纯化的工艺研究[J].中国食品添加剂,2012,2:86-89. [10] 刘艳杰,张健,宋晓秋.大孔树脂纯化紫薯花色苷的研究[J].上海应用技术学院学报:自然科学版,2012,12(1):13-17. [11] 王文平,郭祀远,李琳,等.苯酚-硫酸法测定野木瓜中多糖含量的研究[J].食品科学,2007,28(4):276-279. [12] 王孝平,邢树礼.考马斯亮蓝法测定蛋白含量的研究[J].天津化工,2009(3):40-42. [13] Jungmin L,Durst RW,Wrolstad RE.Determination of Total Monomeric Anthocyanin Pigment Content of Fruit Juices,Beverages,Natural Colorants,and Wines by the p H Differential Method:Collaborative Study[J].Journal of AOAC International,2005,88(5):1269-1278.
[14] 谢捷,刘小景,朱兴一,等.壳聚糖澄清甜叶菊水提液及其澄清机理探讨[J].食品科学,2011,32(20):1-6. [15] 陈钰,潘晓琴,钟振声,等.马铃薯淀粉加工废水中超滤回收马铃薯蛋白质[J].食品研究与开发,2010,31(9):37-41. [16] Chandrasekhar J,Madhusudhan MC,Raghavarao KSMS.Extraction of anthocyanins from red cabbage and purification using adsorption[J].Food and Bioproducts Processing,2012,90(4):615-623.
[17] 朱洪梅,韩永斌,顾振新,等.大孔树脂对紫甘薯色素的吸附与解析特性研究[J].农业工程学报,2006,22(5):153-156. [18] 米聪.紫薯中花色苷的提取纯化及稳定性研究[D].厦门:集美大学,2014.
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