紫薯花色苷的超滤和树脂联用纯化工艺
Purification of anthocyanins from purple sweet potato by combination of ultrafiltration and macroporous resin
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摘要: 利用超滤和大孔树脂联用的方法对紫薯花色苷粗提液进行了纯化,考查了不同截留分子量超滤膜对花色苷粗提液的纯化效果。结果表明:截留分子量为10 ku的膜可有效透过花色苷和截留大分子物质,花色苷的透过率为86.54%,蛋白质截留率为93.67%,透过液的可溶性固形物含量降低了14.29%,透光率提高了23.78%。通过对6种不同极性的大孔吸附树脂对超滤透过液中花色苷的吸附及解吸能力的考查,发现LS-305的吸附性能较好。其动态吸附及解吸条件为:上样流速5.2 BV/h,上样量35 BV;60%乙醇洗脱体积为9 BV可洗脱完全。花色苷产品色价由纯化前的1.59提高到纯化后的32.89。HPLC分析表明超滤和树脂联用纯化方法未对紫薯花色苷成分造成影响。Abstract: The anthocyanins extract from purple sweet potato was purified using ultrafiltration and macroporous resin. The effect of different molecular weight cutoff( MWCO) ultrafiltration membranes to remove the macromolecular substances was investigated. The results showed that the 10 ku membrane could effectively remove the macromolecular substances in the extract. The protein entrapment rate was 93.67%,and the anthocyanins through rate was 86.54%.The soluble solids content was reduced by 14.29%,and light transmittance increased by 23.78%.The adsorption and desorption ability for anthocyanins of six kind of macroporous resins were evaluated.It turned out that LS- 305 was the most suitable resin for anthocyanins. The dynamic adsorption and desorption conditions as follows: flow rate of 5.2 bed volume( BV) / h,the sample volume of 35 BV,60% ethanol used to elute the anthocyanins,and volume of 9 BV could elute anthocyanins completely.As a result,the color value of the anthocyanins from purple sweet potato increased from 1.59 to 32.89 after purification.HPLC analysis showed that ultrafiltration and macroporous resin did not affect the anthocyanins component.