亨氏马尾藻醇提取物及其不同溶剂萃取物的抗氧化活性研究

吴慧; 卢虹玉; 李梅婷; 章超桦

doi:10.13386/j.issn1002-0306.2016.10.026

亨氏马尾藻醇提取物及其不同溶剂萃取物的抗氧化活性研究

广东海洋大学食品科技学院,广东省水产品加工与安全重点实验室,广东普通高等学校水产品深加工重点实验室,国家贝类加工技术研发分中心(湛江),南海生物资源开发与利用协同创新中心

基金项目:

国家“863”项目(2013AA092902)；

详细信息

作者简介:
吴慧(1990-),硕士研究生,研究方向:海洋生物资源利用,E-mail:wh-fly@outlook.com。;

章超桦(1956-),博士,教授,研究方向:水产品及高值化利用,E-mail:zhangch2@139.com。;

中图分类号: TS254.5
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出版历程
- 收稿日期: 2015-10-21

Antioxidant properties of ethanol extract and its different solvent fractions from Sargassum henslowianum

College of Food Science and Technology,Guangdong Ocean University,Guangdong Province Key Laboratory of Aquatic Products Processing and Safety,Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution,National Research and Development Branch Center for Shellfish Processing( Zhanjiang) ,South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center

摘要

摘要: 研究了亨氏马尾藻95%乙醇提取物不同溶剂级分的体外抗氧化活性。采用Folin-Ciocalteu法测定亨氏马尾藻醇提取物（总提取物）以及石油醚萃取物、乙酸乙酯萃取物、正丁醇萃取物和水萃取物五个部位的总酚含量,并通过DPPH·、ABTS⁺·清除及ORAC三种方法评价五种提取物的抗氧化活性。结果表明,乙酸乙酯萃取物的总酚含量最高,为（12.71±0.02）mg GAE/g（没食子酸当量）。在不同抗氧化测定方法中,亨氏马尾藻醇提取物及其不同溶剂萃取物均表现出不同程度的抗氧化活性。其中,乙酸乙酯萃取物抗氧化活性较强,其DPPH和ABTS半数清除率分别为（0.89±0.01）mg/m L和（0.73±0.03）mg/m L,ORAC值达到（1803.78±81.37）μmol TE/g。
- 亨氏马尾藻 /
- 抗氧化活性 /
- ORAC /
- 总酚
Abstract: In this research,antioxidant activitiy of different fractions from ethanol crude extracts of S. henslowianum was investigated in vitro. The total phenolic contents of the 95% ethanol crude extracts and their four solvent fractions( viz.,petroleum ether( PE),ethyl acetate( EA),n- butanol and aqueous) were determined by Folin-Ciocalteu,and their antioxidant properties were evaluated using three assays including 1,1- Diphenyl- 2-picrylhydrazyl radical 2,2- Diphenyl- 1-( 2,4,6- trinitrophenyl) hydrazyl( DPPH),2,2'- Azinobis-( 3-ethylbenzthiazoline-6- sulphonate( ABTS) radical scavenging and oxygen radical absorbance capacity.The results showed that EA fraction had the highest content of total phenolics( 12.71 ± 0.02 mg gallic acid equivalents / g). All five fractions from S. henslowianum exhibited the antioxidant activity. EA fraction exhibited strongest antioxidant activities among all the fractions,which the values of IC_(50) based on DPPH· and ABTS+·were( 0.89 ± 0.01) mg /m L and( 0.73 ± 0.03) mg / m L respectively,and the values of ORAC was( 1803.78 ± 81.37) μmol Trolox equivalents / g.
- S.henslowianum /
- antioxidant activitity /
- ORAC /
- phenols

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