坛紫菜木瓜蛋白酶水解肽的抗肿瘤活性研究

白露; 范晓丹; 张学武

doi:10.13386/j.issn1002-0306.2016.09.061

坛紫菜木瓜蛋白酶水解肽的抗肿瘤活性研究

华南理工大学食品科学与工程学院

基金项目:

广东省省级科技计划项目(2014A020208017)；广东省海洋渔业科技推广专项科技攻关与研发项目(A201301B04)；

详细信息

作者简介:
白露(1990-),女,硕士,主要从事抗肿瘤功能食品的研究,E-mail:bailuhr1990@126.com。;

张学武(1963-),男,博士,教授,主要从事抗肿瘤功能食品的研究,E-mail:snow_dance@sina.com。;

中图分类号: TQ464.7
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出版历程
- 收稿日期: 2015-11-15

Study on the anti- tumor activity of papain hydrolysis peptides derived from Porphyra haitanesis

College of Food Science and Technology,South China University of Technology

摘要

摘要: 坛紫菜是一种重要的经济海藻,营养价值极高。为了探究坛紫菜中的抗肿瘤活性多肽,本研究以坛紫菜粉末为原料,采用反复冻融法和超声波破碎法相结合提取蛋白,使用木瓜蛋白酶对粗蛋白进行酶解。初酶解物在MTT细胞增殖检测结果的指引下,结合超滤、葡聚糖凝胶Sephadex G-15的分离纯化和MALDI-TOF质谱分析,最终筛选出具有较强抗肿瘤活性的多肽。结果显示经木瓜蛋白酶酶解物（设为P,分子量0³ ku）分离纯化得到的多肽组份P2,包含5个多肽,对乳腺癌MCF-7和肝癌细胞Hep G-2的IC50值分别是63.64μg/m L和59.09μg/m L,其抗肿瘤活性均显著高于阳性对照5-氟尿嘧啶（对MCF-7和Hep G-2的IC50值分别是195.45μg/m L和122.72μg/m L）,组份P3包含11个多肽,对肝癌细胞Hep G-2的IC50值是209.09μg/m L,也具有一定的选择性抑制作用。
- 坛紫菜 /
- 酶解 /
- 肽 /
- 抗肿瘤 /
- 活性
Abstract: Porphyra haitanesis is an important economic algae with abundant nutrition.To explore the anti- tumor peptides from Porphyra haitanesis,the protein extracted with coalition of repeated freeing and melting and ultrasonic- extraction were hydrolyzed by papain.The obtained proteolytic peptides were separated and purified by ultrafiltration,Sephadex G- 15 and MALDI- TOF- MS,the whole process was guided by MTT method.Results showed that the component P2 which contained 5 peptides purified from papain hydrolysates( called P,molecular weight 0~3 ku) had a strong anti- proliferation effect on both MCF-7 and Hep G-2 cells,the IC50 were 63.64 μg / m L and 59.09 μg / m L,respectively,both which were superior to the positive control 5- fluorouracil( IC50 of MCF-7 and Hep G- 2 were 195.45 μg / m L and 122.72 μg / m L,respectively).While the component P3 which contained 11 peptides also had a certain selective anti- proliferation effect on Hep G-2 cells with an IC50 of 209.09 μg / m L.
- Porphyra haitanesis /
- enzymatic hydrolysis /
- peptide /
- anti-tumor /
- activity

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