高效液相色谱测定饲料中色氨酸含量方法的研究

Study on determination of tryptophan in feedstuff by high performance liquid chromatography

摘要: 建立了饲料中色氨酸的高效液相色谱分析方法。饲料样品通过氢氧化锂提取,采用C18色谱柱,醋酸盐缓冲液和甲醇为流动相等度洗脱,二级阵列管检测器280 nm波长下检测。实验结果表明:标准曲线在25~500 mg/L范围内线性良好,相关系数r=0.9998,加标回收率为88.33%~105.04%,相对标准偏差在2.80%~5.97%之间,饲料中色氨酸检出限为18.3 mg/kg。本方法准确可靠,适用于饲料中色氨酸的测定。

Abstract: A method for the determination of tryptophan in feedstuff was established by high performance liquid chromatography.The samples were extracted with lithium hydroxide solution.The analysis was carried out on a C18 column with acetate buffer and acetonitrile in gradient elution.The analytes were detected with DAD detector,at280 nm as detection wavelength.The linear ranges was good from 25 mg / L to 500 mg / L with correlation coefficient0.9998.The standard addition recoveries were 88.33% ~ 105.04% and the relative standard dviation( RSD) were2.80% ~5.97%.The detection limit of tryptophan was 18.3 mg / kg.The established methods were proved accurate and reliable,which can be used for the detection of tryptophan in feedstuff.