Optimization of fermentation conditions for extracellular polysaccharide production by streptococcus thermophilus with response surface methodology
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摘要: 本实验采用响应面法（RSM）对影响嗜热链球菌（Q4）产胞外多糖（EPS）的发酵条件进行了优化。以EPS含量为指标,通过单因素实验初步得到Q4产EPS的发酵条件;采用Placket-Burman（PB）设计法从以上单因素中筛选出显著因素,对显著因素进行Box-Behnken（BB）中心组合实验设计优化,建立EPS含量与各影响因素的回归方程,获得Q4产胞外多糖的最佳发酵条件为:初始p H6.8、发酵时间33 h、发酵温度41℃。验证实验结果表明,在此条件下胞外多糖的产量可达268.42 mg/L,比优化前的EPS含量（89.43 mg/L）提高了3倍。Abstract: The quantitative effects of fermentation conditions on the yield of Streptococcus thermophilus( Q4)extracellular polysaccharide were optimized using response surface methodology( RSM).The EPS content as an index,fermentation conditions of EPS produced by Q4 was preliminary obtained by single factor experiment.Then significant factors were screened from the above the single factors according to the Placket- Burman( PB) design method and were optimized using Box- Behnken design.And the EPS content as the response values,obtaining the response surface figure,the optimal fermentation conditions on the yield of( Q4) extracellular polysaccharide were determined: initial p H6.8,fermentation time 33 h and fermentation temperature 41 ℃.Under the optimal conditions the corresponding response value for extracellular polysaccharide production was 268.42 mg / L,compared with the previous EPS content( 89.43 mg / L),which was increased 3 times.
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[1] 李志成,闫亚美,张连斌,等,保加利亚乳杆菌和嗜热链球菌产酸产黏特性研究[J].中国乳品工业,2006,05:8-10. [2] Schellhaass S M,Morris H A.Rheological and scanning electron microscopic examination of skim milk gels obtained by fer-menting with ropy and non-ropy strains of lactic acid bacteria[J].Food Microstruct,1985,4(2):279-287.
[3] Yasui H,Shida K,Matsuzaki T,et al.Immunomodulatory function of lactic acid bacteria[J].Antonie Van Leeuwenhoek,1999,76(1):383-389.
[4] Lin,C-J,Pehkonen SO.HKONEN S Q.The chemistry of atnospheric mercury:A review[J].Atnospheric Enviroment1991.33(13):2067-2079.
[5] Mende S,Peter M,Bartels K,et al.Addition of purified exopolysaccharide isolates from S.thermophilusto milk and their impact on the rheology of acid gels[J].Food Hydroeolloids,2013,32(1):178.
[6] 黄云鹏,姜淑娟,钱方,等.高产胞外多糖嗜热链球菌的诱变育种研究[J].食品工业,2012,33(6):43-45. [7] Patricia Ruas-Madiedo,C G de los Reyes-Gavilan.Invited Review:Methods for the Screening,Isolation,and Characterization of Exopolysaccharides Produced by Lactic Acid Bacteria[J].Journal of Dairy Science,2005,88(3):843-856.
[8] 曹文海,任国谱.嗜热链球菌的检验培养基(M17)的改良[J].中国乳业,2006,1:46-48. [9] 李全阳,夏文水,张进山,等.酸乳中乳酸菌胞外多糖的分离与纯化[J].食品与发酵工业,2005,31(10):139-141. [10] 萨如拉,芒来,赵娜,等.产胞外多糖乳杆菌的菌种鉴定及其发酵条件优化[J].食品科技,2014,39(8):69-72. [11] 唐血梅,李海英,赵芳,等.新疆酸马奶中高产胞外多糖乳酸菌筛选鉴定及培养条件优化研究[J].新疆农业科学,2012,49(8):1540-1545. [12] Jolly L,Stingele.Molecular organization and functionality of exopolysaccharide gene clusters in lactic acid bacteria.Int.Dairy[J].Current Opinion in Biotechnology,2001,11(9):733-746
[13] Cheirrsilp B,Shimizu H,Shioya S.Modeling and optimisation of environmental conditions for kefiran production by Lactobacillus kefn-anofaciens[J].Applied Microbiology and Biotechnology,2001,57:639-646.
[14] Ai L Z,Zhang H,Guo B H,et al.Optimization of Culture Conditions for Exopolysaccharide Production by Lactobacillus Casei Lc2w[J].Milchwissenschaft-Milk Science International,2006,61(4):374-7.
[15] 贾建波,严学伟.产粘乳酸菌的筛选及其产粘多糖条件的研究[C].第三届中国乳业科技大会论文集,2006:185-192. [16] Gorska-Fraczek S,Sandstrom C,Kenne L,et al.Structural studies of the exopolysaccharide consisting of a nonasaccharide repeating unit isolated from Lactobacillus rhamnosus KL37B[J].Carbohyd Res.2011,346:2926-2932.
[17] 王辑,田政,赵笑,等.藏灵菇发酵产胞外多糖的影响因素及其应用研究[J].食品工业科技,2015,36(18):203-208. [18] Salvador A,Fiszman S M.Textural and sensory characteristics of whole and skimmed flavored set-type yogurt during long storage[J].Journal of Dairy Science,2004,87(12):4033-4041.
[19] 刘慧,熊利霞,韩睿,等.藏灵菇源干酪乳杆菌KL1高产胞外多糖发酵条件的优化研究[J]l.中国农学通报,2008,24(11):117-121. [20] 王英,周剑忠,黄开红,等.益生菌干酪乳杆菌FM10-3产胞外多糖培养条件的优化[J].2014,2:9-12. [21] 宫宇飞,乔红萍.内生枯草芽抱杆菌EIR-J发酵条件的优化[J].西北农业学报,2008.17(1):61-64. [22] 易周军,朱文学,马海乐,等.牡丹籽油超声波辅助提取工艺的响应面法优化[J].农业机械学报,2009,40(6):103-110.
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