基于HiCapt Benzo固相萃取前处理的HPLC-FLD法检测油茶籽油中苯并（a）芘

李圣陶; 龚吉军; 吴敏; 唐静

doi:10.13386/j.issn1002-0306.2016.08.007

基于HiCapt Benzo固相萃取前处理的HPLC-FLD法检测油茶籽油中苯并（a）芘

李圣陶^1,2,
龚吉军^1,3,
吴敏⁴,
唐静^1,3

1. 中南林业科技大学食品科学与工程学院
2. 北海市农产品质量检测中心
3. 稻谷及副产物深加工国家工程实验室
4. 厦门出入境检验检疫局科学研究院

基金项目:

湖南省科技计划项目(2014NK3054)；粮油深加工与品质控制湖南省2011协同创新中心开放课题(湘教通[2013]448号)；中南林业科技大学“十二五”专业综合改革试点项目(2013-6)；国家质检总局科技计划项目(2012IK192)；

详细信息

作者简介:
李圣陶(1986-),男,硕士研究生,研究方向:食品质量与安全,E-mail:349065553@qq.com。;

龚吉军(1972-),男,博士,教授,研究方向:食品质量与安全,食品天然保鲜剂,E-mail:jijungong2007@163.com。;

中图分类号: O657;TS225.16
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出版历程
- 收稿日期: 2015-08-18

A detection method over benzo(a) pyrene in Camellia oleifera Abel seed oil by HPLC-FLD based on Hi Capt Benzo solid-phase extraction pretreatment

College of Food Science and Engineering,Central South University of Forestry and Technology
Quality Inspection Center of Agricultural Products of Beihai City
National Engineering Laboratory for Rice and Byproducts Processing
Institute,Xiamen Entry-Exit Inspection and Quarantine Bureau

摘要

摘要: 为了建立一种基于Hi Capt Benzo固相萃取快速前处理的,更灵敏、准确的高效液相色谱-荧光检测法,以用于油茶籽油中苯并（a）芘的检测。油茶籽油样品以正己烷溶解后,用Hi Capt Benzo专用固相萃取柱净化。检测条件为:LC-PAHs专用色谱柱;50%乙腈-50%水为流动相,流速1.0 m L/min;荧光检测器;外标法定量。研究结果表明,苯并（a）芘标准曲线在0.1²00 ng/m L范围内,线性关系好（R²=0.9993）;检测限很低（0.0213⁰.0246μg/kg）。该法用于4个油茶籽油样品加标检测,回收率为93.06%¹04.52%,相对标准偏差（RSD）为2.12%³.98%。本文所建固相萃取-高效液相色谱（带荧光检测器）法前处理简单,结果准确可靠,是油茶籽油苯并（a）芘高效检测的适宜方法。
- 油茶籽油 /
- 苯并(a)芘 /
- 液相色谱 /
- 固相萃取 /
- 检测
Abstract: This study was aimed to establish a highly efficient method of high-performance liquid chromatography with fluorescence detection(HPLC-FLD) based on Hi Capt Benzo solid-phase extraction time-saving pretreatment for benzo(a) pyrene [B(a)P] detection in Camellia oleifera Abel seed oil(COASO). Samples of COASO were dissolved in hexane,and purified by Hi Capt Benzo solid phase extraction(SPE) column. The detection conditions were as followings:LC-PAHs chromatographic column,50% acetonitrile-50% pure water as mobile phase,flow rate 1.0 m L/min,fluorescence detector,quantification by external standard method. The results showed that standard calibration curve was linear over the concentration range of 0.1~200 ng/m L,with the regression efficiency(R²) of 0.9993. Furthermore,the detection limit was 0.0213~0.0246 μg/kg. A recovery range of 93.06%~104.52%and a relative standard deviation(RSD) range of 2.12%~3.98% was exhibited when the method was employed to detect four samples of COASO added with [B(a)P] standand. It was suggested that this detection method of SPE-HPLC-FLD established in the study was a suitable one for [B(a)P] detection in COASO,for its obvious advantages,such as simpler pretreatment,more sensitive and more efficient.
- Camellia oleifera Abel seed oil(COASO) /
- B(a)P /
- high-performance liquid chromatography /
- solid phase extraction /
- detection

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