Abstract: This study was aimed to establish a highly efficient method of high-performance liquid chromatography with fluorescence detection(HPLC-FLD) based on Hi Capt Benzo solid-phase extraction time-saving pretreatment for benzo(a) pyrene B(a)P detection in Camellia oleifera Abel seed oil(COASO). Samples of COASO were dissolved in hexane,and purified by Hi Capt Benzo solid phase extraction(SPE) column. The detection conditions were as followings:LC-PAHs chromatographic column,50% acetonitrile-50% pure water as mobile phase,flow rate 1.0 m L/min,fluorescence detector,quantification by external standard method. The results showed that standard calibration curve was linear over the concentration range of 0.1~200 ng/m L,with the regression efficiency(R²) of 0.9993. Furthermore,the detection limit was 0.0213~0.0246 μg/kg. A recovery range of 93.06%~104.52%and a relative standard deviation(RSD) range of 2.12%~3.98% was exhibited when the method was employed to detect four samples of COASO added with B(a)P standand. It was suggested that this detection method of SPE-HPLC-FLD established in the study was a suitable one for B(a)P detection in COASO,for its obvious advantages,such as simpler pretreatment,more sensitive and more efficient.