杂粮蛹虫草菌丝共生体中虫草素的优化提取及测定

常正姣; 韩萍; 刘利娥; 许艳丽; 蒋永红; 王文宁

doi:10.13386/j.issn1002-0306.2016.07.037

杂粮蛹虫草菌丝共生体中虫草素的优化提取及测定

郑州大学公共卫生学院

详细信息

作者简介:
常正姣(1988-),女,在读硕士研究生,研究方向:营养与疾病,E-mail:bluesysbb@live.cn。;

韩萍(1960-),男,博士,教授,研究方向:营养与疾病,E-mail:hp999@zzu.edu.cn。;

中图分类号: TS218
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出版历程
- 收稿日期: 2015-08-06

Optimizing extraction and determination of cordycepin in multigrain cordyceps militaris hyphae symbionts

College of Public Health,Zhengzhou University

摘要

摘要: 目的:探讨杂粮蛹虫草菌丝共生体中虫草素的最优提取条件,并比较了不同光照时长对虫草素含量的影响,为其工艺条件的优化和综合利用提供依据。方法:采用单因素结合响应曲面法优化虫草素的水浴提取条件,高效液相色谱法测定虫草素含量。结果:最优水浴提取条件为:浸提时间6.59 h、浸提温度81.9℃、料液比1∶90,此条件下虫草素含量为6.0143 mg/g;不同光照时长条件下虫草素含量为3.8988~11.9629 mg/g,不同光照时长间差异具有统计学意义(p=0.000)。结论:实验确定了虫草素水浴提取的最优条件,并得出不同光照时长对虫草素含量有显著影响,为进一步优化杂粮蛹虫草菌丝共生体的培养条件提供参考依据。
- 杂粮蛹虫草菌丝共生体 /
- 虫草素 /
- 响应面法 /
- 高效液相色谱法 /
- 光照时长
Abstract: Object: To explore the optimal extraction conditions of cordycepin in multigrain cordyceps militaris hyphae symbionts,the effect of varied lighting duration on cordycepin was compared,the purpose was to provide a basis for the optimization of its technological conditions and comprehensive utilization.Methods: The conditions of waterbath extraction of cordycepin were determined by single factor experiments combined with response surface methodology,the content of cordycepin was determined by high performance liquid chromatography,SPSS 17.0software was applied for statistical analysis. Results: The results showed that the optimal extraction conditions were: extraction time 6.59 h,extraction temperature 81.9 ℃,solid- liquid ratio 1∶90.The contents of cordycepin were6.0143 mg / g under this condition. The contents of cordycepin were 3.8988 ~ 11.9629 mg / g in different lighting duration and were significantly different( p = 0.000).Conclusions: The optimal conditions of waterbath extraction of cordycepin were determined and the contents of cordycepin were significantly different under varied lighting duration,which can provide the reference basis for further optimization of culture conditions of multigrain cordyceps militaris hyphae symbionts.
- multigrain cordyceps militaris hyphae symbionts /
- cordycepin /
- response surface methodology(RSM) /
- high performance liquid chromatography(HPLC) /
- lighting duration

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