红松松仁膜衣提取物体外抗氧化活性研究

张晓茹; 李星; 王彬; 王增凯; 徐红艳

doi:10.13386/j.issn1002-0306.2016.06.020

红松松仁膜衣提取物体外抗氧化活性研究

延边大学农学院
延吉秀爱食品有限公司

基金项目:

吉林省科技发展计划项目(20150204063NY)；延边大学大学生创新计划项目(ydbksky2015244)；

详细信息

作者简介:
张晓茹(1991-),女,大学本科,研究方向:天然活性物质功能作用,E-mail:919958472@qq.com。;

徐红艳(1975-),女,博士,讲师,研究方向:天然活性物质分离与功能性食品,E-mail:xuhongyan@ybu.edu.cn。;

中图分类号: TS209
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出版历程
- 收稿日期: 2015-06-11

Antioxidant activities in vitro of pine nut coated-film extracts from Pinus koraiensis

Agricultural College of Yanbian University
Yanji Shoei Foods Co.,Ltd.

摘要

摘要: 本文旨在探明长白山红松松仁膜衣提取物的体外抗氧化活性。分别采用60%乙醇和50%丙酮为提取剂,提取物冻干后,用分光光度法测其总多酚和总黄酮纯度,研究其自由基清除能力和还原铁离子的能力。结果表明,松仁膜衣乙醇提取物总多酚纯度为39.02%,总黄酮纯度为10.19%;丙酮提取物总多酚纯度为44.09%,总黄酮纯度为14.59%。红松松仁膜衣提取物清除ABTS⁺·、DPPH·、羟自由基（·OH）的能力和还原铁离子能力都呈现一定的剂量效应关系,且对DPPH·的清除作用和还原铁离子能力强于BHT;丙酮提取物对ABTS⁺·和·OH的清除能力显著高于乙醇提取物,表明丙酮提取物的抗氧化能力强于乙醇提取物。同时,在实验范围内,红松松仁膜衣提取物的抗氧化能力与其总多酚、总黄酮的纯度存在正相关性。
- 红松 /
- 松仁膜衣 /
- 抗氧化
Abstract: It was to find out the antioxidant activities of Pinus koraiensis pine nut coated-film from Changbai Mountain. 60% ethanol and 50% acetone were used,and the extracts were obtained by vacuum freeze-drying.The purity of total polyphenol and total flavonoids of the extracts were measured by spectrophotometry. The free radical scavenging abilities and reducing iron power also were studied in this experiment. The results showed that polyphenol purity of pine nut coated-film ethanol extract was 39.02%,the flavonoid purity was10.19%. The polyphenol purity of acetone extract was 44.09%,the flavonoid purity was 14.59%. The scavenging capacity of pine nut coated-film extract on ABTS⁺·,DPPH· and hydroxyl radical(·OH) was of dose-dependent relationship including the ability of reducing iron power. Its scavenging effects on DPPH· and the ability of reducing iron power were better than that of BHT. And scavenging effects of acetone extracts on DPPH· and·OH were notable better than that of ethanol extracts. These results showed that the antioxidant capacity of acetone extracts was higher than that of ethanol extracts. At the same time,there was positive correlation between the antioxidant capacity of Korean pine nut coated-film extracts and the purity of total polyphenol and total flavonoids in experiment extent.
- Pinus koraiensis /
- pine nut coateded-film /
- antioxidant activity

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