Determination of lobetyolin and syringin in Codonopsis Radix from different places by HPLC
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摘要: 建立高效液相色谱法同时测定不同产地党参中党参炔苷和丁香苷含量的方法。采用Thermo scientific C18色谱柱（4.6 mm×250 mm,5μm）,以乙腈和水为流动相进行梯度洗脱,流速为1.0 m L/min,检测波长266 nm,柱温为25℃。实验结果表明,党参炔苷与丁香苷色谱峰分离良好。二者分别在10200μg/m L与0.510μg/m L范围内与峰面积呈良好的线性关系。平均加样回收率分别为100.65%与98.78%。党参炔苷和丁香苷在10个不同产地的党参中含量分别介于0.19631.4532 mg/g与0.00510.0951 mg/g。该方法操作简便、快速,结果稳定可靠,适用于党参2种主要成分的定量分析。Abstract: To establish a HPLC method for simultaneous determination of lobetyolin and syringin in Codonopsis Radix from different places. The HPLC system consisting of Thermo scientific C18 Column(4.6 mm×250 mm,5 μm) and a gradient elution system of acetonitrile-water as the mobile phase was adopted. The flow rate was set at 1.0 m L/min,the detection wavelength was 266 nm,and the column temperature was 25 ℃. The results indicated that the chromatographic peaks of lobetyolin and syringin could be separated well. The linear ranges for lobetyolin and syringin were 10 ~200 μg/m L and 0.5 ~10 μg/m L,respectively. The average recoveries were100.65% and 98.78%,respectively. The lobetyolin content and syringin content in Codonopsis Radix from ten places was ranged from 0.1963 ~1.4532 mg/g and 0.0051 ~0.0951 mg/g,respectively. The simple,quick,reproducible and reliable method could be used for determination of two main components in Codonopsis Radix.
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Keywords:
- Codonopsis Radix /
- HPLC /
- lobetyolin /
- syringin /
- content assay
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