Extraction of total flavonoids from leaves of Indocalamus using inner ebullition method
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摘要: 目的:确定内部沸腾法提取箬叶总黄酮的优化工艺。方法:以黄酮得率为指标,分别在解吸过程和提取过程的单因素实验基础上,进行正交实验,考察内部沸腾法提取箬叶总黄酮含量的最佳条件,并与回流法、超声波法、微波法进行了比较。结果:内部沸腾法提取箬叶总黄酮最佳条件是在固液比1∶3的90%乙醇溶液中解吸25 min后,在85℃下,在固液比为1∶30的30%乙醇溶液中提取5 min,箬叶总黄酮的得率达到2.05%,异荭草苷含量为1.656 mg·g-1,荭草苷含量为0.454 mg·g-1,与回流法、超声波法和微波法相比,黄酮得率差异不显著（p>0.05）,但乙醇用量少,成本低。Abstract: Objective: To explore the optimum extraction process of total flavonoids from leaves of Indocalamus by inner ebullition method. Methods: Through single- factor experiments of desorption / extraction process and the orthogonal experiments,the optimal extract conditions of the total flavonoids from leaves of Indocalamus by using inner ebullition method was studied. The method was also compared with reflux method,ultrasonic method and microwave method.Results: The optimal desorption conditions were as follows: solid- liquid ratio was 1 ∶ 3,ethanol concentration was 90%,the resolution time was 25 min. The optimal extraction conditions were: solid- liquid ratio was 1∶ 30,the temperature was 85 ℃,ethanol concentration was 30%,the extraction time was 5 min.The yield of total flavonoids was 2.05%,the content of isoorientin and orientin was 1.656,0.454 mg·g-1.Comparing with reflux method,ultrasonic method and microwave method,the inner ebullition method had no significant difference of yield of flavonoids,but lesser amount of ethanol and cheaper cost.
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Keywords:
- inner ebullition /
- total flavonoids /
- Indocalamus
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