响应面优化西兰花中萝卜硫素复合提取工艺

胡翠珍; 李胜; 马绍英; 曹宝臣; 唐斌; 赵生琴; 时振振; 苏利荣; 周文政

doi:10.13386/j.issn1002-0306.2016.04.046

响应面优化西兰花中萝卜硫素复合提取工艺

甘肃省干旱生境作物学重点实验室甘肃农业大学生命科学技术学院
张掖汇通生物科技有限公司
甘肃顺意生物科技有限公司

基金项目:

兰州市科技计划项目(2014-1-183)；教育部新教师基金(20096202120008)；国家大学生创新训练计划(201210733009)；甘肃农业大学本科生科研训练计划(20140824;20140825)；甘肃农业大学盛彤笙科技创新基金(2015)；

详细信息

作者简介:
胡翠珍(1989-),女,硕士研究生,研究方向:天然产物的提取与开发,E-mail:zhonglarkspur@163.com。;

李胜(1970-),男,博士,教授,研究方向:植物细胞工程与次生代谢调控,E-mail:lish@gsau.edu.cn。;

中图分类号: TS255.36
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出版历程
- 收稿日期: 2015-06-14

Response surface optimizes the compound extraction process of sulforaphane from broccoli

Gansu Provincial Key Lab of Arid land Crop Science,College of Life Science and Technology,Gansu Agricultural University
Lanzhou Huitong Bio-Technology Co.,Ltd.
Gansu Shunyi Bio-Technology Co.,Ltd.

摘要

摘要: 以西兰花鲜食部分绿色幼嫩花茎和花蕾为实验材料,利用响应面对添加剂和超声波辅助酶解西兰花提取萝卜硫素工艺进行优化。实验在单因素基础上选取四种添加剂抗坏血酸、EDTA、Mg SO₄、CaCl₂,根据Central Composite中心复合实验设计原理采用四因素三水平的响应面分析法,确定各因素对萝卜硫素提取率的影响程度,获得该四因素最佳提取条件为:抗坏血酸4.659 mmol/L,EDTA 6 mmol/L,Mg SO₄ 8.5 mmol/L,Ca Cl24.0 mmol/L,萝卜硫素理论最大提取率达1858.20μg/g。经实验验证达1813.26μg/g,其相对误差为2.48%,表明该提取工艺参数在西兰花中提取萝卜硫素是可行的。
- 萝卜硫素 /
- 酶解 /
- 添加剂 /
- 单因素 /
- 响应面
Abstract: The green young stems and buds of broccoli were used as experimental material,the enzymolysis condition of sulforaphane extraction from broccoli was optimized by response surface method. According to central composite experiment design principle and using response surface analysis of four factors and three levels,four additives including ascorbic acid,EDTA,Mg SO_4,Ca Cl_2 were chosen on the basis of the single factor to determine the impact of various factors on the extraction rate of sulforaphane. The optimum extraction conditions were as follows:ascorbic acid 4.659 mg/g,EDTA 6 mmol/L,Mg SO_4 8.5 mmol/L,Ca Cl_2 4.0 mmol/L.The theoretical maximum extraction rate of sulforaphane was 1858.20 μg/g,and the value reached 1813.26 μg/g in the experiment,the relative error was 2.48%,which indicated that the extraction process parameter above was feasible in sulforaphane extraction from broccoli.
- sulforaphane /
- enzymolysis /
- additive /
- single factor /
- response surface

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参考文献(20)

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