漆树籽粕多糖的提取工艺优化及抗氧化活性研究

陈虹霞; 王成章; 叶建中; 周昊; 陶冉; 李文君

doi:10.13386/j.issn1002-0306.2016.04.043

漆树籽粕多糖的提取工艺优化及抗氧化活性研究

1. 中国林业科学研究院林产化学工业研究所生物质化学利用国家工程实验室国家林业局林产化学工程重点开放性实验室江苏省生物质能源与材料重点实验室
2. 中国林业科学研究院林业新技术研究所

基金项目:

国家林业局948技术引进项目(2015-4-46)；中国林科院林业新技术所基本科研业务费专项资金(CAFINT2013C03)；国家国际科技合作专项(2014DFR31300)；

详细信息

作者简介:
陈虹霞(1983-),女,博士研究生,助理研究员,主要从事天然产物化学方面的研究,E-mail:shirenyahui@126.com。;

王成章(1966-),男,博士,研究员,主要从事天然产物研究与利用方面的研究,E-mail:wangczlhs@sina.com。;

中图分类号: TQ281
计量
- 文章访问数: 225
- HTML全文浏览量: 33
- PDF下载量: 180
出版历程
- 收稿日期: 2015-08-23

Extraction optimization of crude polysaccharides from the seed cakes of Rhus succedanea L and its antioxidant activity

Institute of Chemical Industry of Forestry Products,CAF Key and Open Lab on Forest Chemical Engineering,SFA
Institute New Technology of Forest,CAF

摘要

摘要: 以脱脂后的漆树籽粕为原料,采用热回流提取漆树籽粕多糖,通过单因素实验和正交实验优化该方法的最佳工艺条件,并进一步研究漆树籽粕多糖对羟自由基和ABTS+自由基的清除能力。结果表明:最佳工艺条件为提取时间2.5 h,料液比1∶20（g∶m L）,提取温度80℃和提取次数2次,此时籽粕多糖的得率为1.561%。漆树籽粕多糖对清除羟自由基和ABTS+自由基的IC₅₀分别为8.515 mg·m L^-1和7.03 mg·m L^-1,当漆树籽粕多糖的浓度为25 mg·m L^-1时,对羟自由基的清除率达到61.5%,当浓度为10 mg·m L-1时,对ABTS+自由基的清除率达到58.4%。体外抗氧化实验表明漆树籽粕多糖抗氧化活性作用明显。
- 漆籽 /
- 籽粕 /
- 多糖 /
- 提取 /
- 抗氧化活性
Abstract: Single factor and orthogonal methodology were used to optimize the extraction conditions of crude polysaccharides from the seed cakes of Rhus succedanea L,then the antioxidant(scavenging hydroxyl and ABTS radicals) of the crude polysaccharides from the seed cakes of Rhus succedanea L were possessed. The optimum extraction conditions were as follows :extraction time 2.5 h,ratio of material to water 1 ∶20(g∶m L),extraction temperature 80 ℃,and extraction times 2. Under the optimized conditions,an experimental yield was 1. 561 %. The IC_(50) values scavenging hydroxyl and ABTS~+radicals of the crude polysaccharides were8.515 mg·m L~(-1) and 7.03 mg·m L~(-1),respectively. The hydroxyl radical scavenging ability of polysaccharides reached 61.5% at 25 mg·m L~(-1). The ABTS+radicals scavenging ability of polysaccharides reached 58.4% at10 mg·m L~(-1). The seed cakes polysaccharides displayed significant antioxidant.
- Rhus succedanea L /
- seed cakes /
- polysaccharides /
- extraction /
- antioxidant activity

HTML全文

参考文献(15)

[1]	董艳鹤,王成章,宫坤,等.漆树资源的化学成分及其综合利用研究进展[J].林产化学与工业,2009,29(B10):225-232.
[2]	Zong A Z,Cao H Z,Wang F S.Anticancer polysaccharides from natural resources:A review of recent research[J].Carbohydrate Polymers,2012,90(4):1395-1410.
[3]	Cho E J,Hwang H J,Kim S W,et al.Hypoglycemic effects of exopolysaccharides produced by mycelial cultures of two different mushrooms Tremella fuciformis and Phellinus baumii in ob/ob mice[J].Applied Microbiology and Biotechnology,2007,75(6):1257-1265.
[4]	Suvakanta D,Narsimha M P,Pulak D,et al.Optimization and characterization of purified polysaccharide from Musa sapientum L.as a pharmaceutical excipient[J].Food Chemistry,2014,149:76-83.
[5]	Abou Zeid A H,Aboutabl E A,Sleem A A,et al.Water soluble polysaccharides extracted from Pterocladia capillacea and Dictyopteris membranacea and their biological activities[J].Carbohydrate Polymers,2014,113:62-66.
[6]	Facchini J M,Alves E P,Aguilera C,et al.Antitumor activity of Pleurotus ostreatus polysaccharide fractions on Ehrlich tumor and Sarcoma 180[J].International Journal of Biological Macromolecules,2014,68:72-77.
[7]	Pakrokh Ghavi P.The extraction process optimization of antioxidant polysaccharides from Marshmallow(Althaea officinalis L.)roots[J].International Journal of Biological Macromolecules,2015,75:51-57.
[8]	董艳鹤,王成章,叶建中,等.漆蜡的提取工艺及其化学成分[J].北京林业大学学报,2010,(4):256-260.
[9]	董艳鹤,王成章,陈虹霞,等.漆蜡的吸附脱色工艺研究[J].林产化学与工业,2011,31(2):91-95.
[10]	陈虹霞,王成章,叶建中,等.漆蜡脂肪酸甲酯磺酸盐的制备及其性能研究[J].中国油脂,2014,39(5):37-40.
[11]	Chung S K,Osawa T,Kawakishi S.Hydroxyl radical scavenging effects of species and scavengers from brown mustard(Brassia nigra)[J].Bioscience Biotehnology and Biochemistry,1997,61:118-123.
[12]	Re R,Pellegrini N,Proteggente A,et al.Antioxidant activity applying and improved ABTS radical cation ecolorization assay[J].Free Radic Biol Med,1999,26(9):1231-1237.
[13]	Chance B,Sies H,Boveris A.Hydroperoxide metabolism in mammalian organs[J].Physiological Reviews,1979,59:527-605.
[14]	Bohn J A,Be Miller J N.(1→3)-β-d-Glucan as biological response modifiers:A review of structure-functional activity relationships[J].Carbohydrate Polymers,1995,28:3-14.
[15]	Miller N J,Rice-Evans C A,Davies M J,et al.A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates[J].Clin Sci,1993,84:407-412.