Optimization on the ultrasonic- assisted extraction of total flavonoids from Iberis amara based on orthogonal experimental design
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摘要: 本文采用正交实验设计法优化了超声波辅助提取屈曲花总黄酮的工艺条件。首先,通过单因素实验研究乙醇浓度、提取时间、料液比和超声波功率对总黄酮得率的影响。然后,利用正交实验设计法优化最佳提取工艺。实验结果表明,乙醇浓度对屈曲花总黄酮得率的影响最大,其次依次为提取时间、料液比、超声波功率。最佳的提取条件为乙醇浓度85%、料液比1∶70、提取时间15 min、超声波功率100 W。在该条件下,总黄酮得率为2.456%。本文为工业化应用提供了参考标准,也为广泛开发利用屈曲花药物资源提供了良好的参考价值。Abstract: Orthogonal experimental design was applied to optimize the extraction process of total flavonoids from Iberis amara using an ultrasonic- assisted extraction method in this study. The effects of ethanol concentration,extraction time,solid- to- liquid ratio and ultrasonic power on the extraction yield of total flavonoids were investigated by single- factor experiment,and then the extraction conditions were optimized by orthogonal experimental design. The results displayed that the ethanol concentration was the most key factor,followed by extraction time,solid- to- liquid ratio and ultrasonic power,in turn.The best extraction conditions were obtained as follows: ethanol concentration 85%,solid- to- liquid ratio 1 ∶ 70,extraction time 15 min and ultrasonic power 100 W.Under these optimized conditions,the yield of total flavonoids from Iberis amara was 2.456%.This study offered not only a reference standard for industrial applications,but also has a promising prospect and value for widely exploitation and utilization of Iberis amara resource.
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Keywords:
- Iberis amara /
- flavonoids /
- ultrasonic-assisted extraction /
- orthogonal experiment
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