Effect of astaxanthin combined with vitamin E on acute alcohol-induced oxidative stress in rats
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摘要: 研究虾青素与维生素E联合作用对酒精诱导氧化应激大鼠血清及组织的影响。将50只雄性Wistar大鼠随机分为5组:对照组（n=10）、模型组（n=10）、虾青素组（n=10,0.6 g·kg-1·d-1）、维生素E组(n=10,0.2 g·kg-1·d-1)、虾青素(0.3 g·kg-1·d-1)及维生素E(0.1 g·kg-1·d-1)联用组（n=10）。连续30 d给予抗氧化剂,末次灌胃12 h后,除对照组外,其余各组灌胃12 m L/kg 50%酒精溶液诱导氧化应激。观察各抗氧化剂组对大鼠的影响。研究表明单独服用虾青素（AX）及维生素E（VE）均可提高血清及心、肝、肾组织的总抗氧化能力（T-AOC）及谷胱甘肽（GSH）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）活性,并且降低血清及心、肝、肾组织的丙二醛（MDA）含量。虾青素组及维生素E组抗氧化效果均未有虾青素及微生素E联用组效果明显。结果表明虾青素与维生素E联用可能具有协同作用,与单一抗氧化剂相比,虾青素与维生素E联用具有更强的抗氧化性,并对急性酒精引起的氧化应激具有明显的保护效果。Abstract: The present study was conducted to investigate the combination effects of astaxanthin(AX) combined with vitamin E on reversing acute alcohol-induced oxidative stress in rats. Total 50 male Wistar rats were selected and randomly divided into control group(n=10),model group(n=10),AX group(n=10,0.6 g·kg-1·d-1),V_E group(n=10,0.2 g·kg~(-1)·d~(-1)) and AXVE(0.3,0.1 g·kg~(-1)·d~(-1)) group(n=10). Following a 30 consecutive with antioxidative drugs treatment,model group,AX group,V_E group and AXVE group(n=10) oral received a single dose of alcohol(12 m L·kg~(-1)) 12 hours after the last treatment. Anti-oxidative indices of serum and organs samples were collected and detected 6 hour after alcohol treatment. In comparison with model group,AX,V_E and the combination reagents of AX and VEenhanced the activities of the total anti-oxidative capacity(T-AOC),glutathione(GSH),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px) from serum,heart,liver and kidney samples, while inhibited the malondialdehyde( MDA) levels in the serum, heart, liver and kidney,with a predominant effects of complex treatment. The findings indicated that compared to treatment of AX or V_E alone,AX combined with V_E together mitigated oxidative damage effectively which induced by acute alcohol exposure. It might due to the synergistic actions of AX combined with V_E.
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Keywords:
- astaxanthin /
- vitamin E /
- acute alcohol exposure /
- oxidative stress /
- rats
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