不同条件下萃取花生蛋白前后AOT/异辛烷反胶束粒径与萃取率相互关系变化的研究

王丽敏; 陈复生; 刘昆仑; 殷丽君

doi:10.13386/j.issn1002-0306.2016.02.038

不同条件下萃取花生蛋白前后AOT/异辛烷反胶束粒径与萃取率相互关系变化的研究

河南工业大学粮油食品学院

基金项目:

国家自然科学基金项目(21176058)； 863项目(2013AA102208)；郑州市创新型科技人才队伍建设工程(ISTTCPZZC)；河南省成果转化资金项目(132201610014)；

详细信息

作者简介:
王丽敏(1989-),女,硕士研究生,研究方向:食品蛋白质资源开发及利用,E-mail:wanglm2000@126.com。;

陈复生(1963-),男,教授,研究方向:食品蛋白质资源开发与利用,E-mail:fushengc@aliyun.com。;

中图分类号: TS201.21
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出版历程
- 收稿日期: 2015-07-20

Changes of relationship between the sizes of reverse micelles before and after extraction of peanut protein and their extraction rates

College of Food Science and Technology,Henan University of Technology

摘要

摘要: 研究了在不同条件下萃取花生蛋白前后丁二酸二异辛酯磺酸钠(AOT)/异辛烷反胶束粒径变化及前萃率和后萃率变化,考察了反胶束粒径变化与前萃率和后萃率之间相互关系的变化规律。结果表明,KCl浓度增加,使粒径减小,前萃率也相应降低;在0.04~0.09 g/m L范围内,AOT浓度越大,空胶束及实胶束粒径越大,但是前萃率降低,而后萃率则相反;缓冲液p H对空胶束粒径影响不显著,但通过静电相互作用影响实胶束粒径和萃取率;不同的离子种类对反胶束粒径及花生蛋白的萃取影响差异显著,其中含Mg Cl2的缓冲溶液在添加量为0.03 m L/m L前萃率达到最大值98.55%±0.60%。反胶束粒径与萃取率之间的关系会受到许多因素的影响,不同因素影响下,二者之间的关系不同;并得出反胶束前萃花生蛋白的模型符合"水壳模型"。
- 反胶束 /
- 花生蛋白 /
- 粒径 /
- 前萃率 /
- 后萃率
Abstract: This study evaluated the sizes of reverse micelles before and after the extraction of peanut protein by AOT/isooctane reverse micelles and investigated the relationship between the size change and extraction rates.The results showed that increasing the concentration of KCl,the particle sizes and forward extraction rates decreased. And increasing the concentration of AOT in the range of 0.04 ~0.09 g/m L,the empty reverse micelles and full reverse micelles increased,forward extraction rates increased,while the backward extraction rates were on the contrary. The p H had no significant influence on empty reverse micelles,but could influence full reverse micelles sizes and extraction rates by electrostatic interaction. The ion species had significant effects on the sizes of reverse micelles and extraction of peanut protein,when the addition amount of buffer solution containing Mg Cl2 was 0.03 m L/m L,the forward extraction rate reached the peak of 98.55%±0.60%. The relationship between reverse micelles sizes and extraction rates could influenced by many factors,and their relation varied under different conditions. The model of the forward extraction process was “water-shell model”.
- reverse micelle /
- peanut protein /
- size /
- forward extraction rate /
- backward extraction rate

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参考文献(17)

[1]	LIU Yang,D X a S Y.New Development of Reverse Micelles and Applications in Protein seperation and refolding[J].Chinese Journal of Chemical Engineering,2008,16(9):949-955.
[2]	Leser M E,Luisi P L,Paimieri S.The use of reverse micelles for the simultaneous extraction of oil and proteins from vegetable meal[J].Biotechnology and Bioengineering,1989,34(9):1140-1146.
[3]	Guanhao B.Effects of different factors on the forward extraction of soy protein in reverse micelle systems[J].African Journal of Biotechnology,2012,11(28):7247-7257.
[4]	Gochman-Hecht H,Bianco-Peled H.Structure modifications of AOT reverse micelles due to protein incorporation[J].Journal of colloid and interface science,2006,297(1):276-283.
[5]	布冠好,刘海远,陈复生,等.AOT/异辛烷反胶束体系特性及萃取大豆蛋白的研究[J].河南工业大学学报:自然科学版,2012,33(6):7-12.
[6]	刘晓燕,闫杰.反胶束体系在蛋白质萃取中应用的研究进展[J].食品工业科技,2010(4):374-376.
[7]	Zhao X,Wei Z,Du F,et al.Effects of surfactant and salt species in reverse micellar forward extraction efficiency of isoflavones with enriched protein from soy flour[J].Applied biochemistry and biotechnology,2010,162(7):2087-2097.
[8]	蔡宝玉,许林妹,彭远宝.反胶束萃取大豆蛋白的研究[J].中国油脂,2004,29(6):37-39.
[9]	磨礼现,陈复生,杨宏顺.反胶束溶液萃取大豆蛋白前萃工艺的研究[J].食品科学,2004,25(3):93-96.
[10]	Koyamatsu Y,Hirano T,Kakizawa Y,et al.p H-responsive release of proteins from biocompatible and biodegradable reverse polymer micelles[J].Journal of Controlled Release,2014,173(2):89-95.
[11]	韦一能,陈元发,陈全斌,等.花生蛋白质的主要种类和等电点的研究[J].广西科学,1995,2(4):1-5.
[12]	杨趁仙,陈复生,刘昆仑,等.含酶反胶束体系后萃花生蛋白工艺优化研究[J].粮食与油脂,2014,27(8):41-44.
[13]	赵涛涛,宫厚健,徐桂英,等.阴离子表面活性剂在水溶液中的耐盐机理[J].油田化学,2010,27(1):112-118.
[14]	滕弘霓,王飞,孙美娟,等.盐对正负离子表面活性剂双水相性质的影响[J].化学学报,2005,63(17):1570-1574.
[15]	Nishii Y,Kishi Y,Ito M,et al.Effect of cation species on equilibrium of reversed micellar extraction of lysozyme[J].Separation and Purification Technology,2004,37(2):161-167.
[16]	Kinugasa T,Kondo A,Mouri E,et al.Effects of ion species in aqueous phase on protein extraction into reversed micellar solution[J].Separation and Purification Technology,2003,31(3):251-259.
[17]	牛巍,侯彩云,祝晓芳,等.三氯乙酸沉淀法与硫酸铜沉淀法在液态奶蛋白质检测中适用性研究[J].中国乳品工业,2008,36(9):59-61.

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