Changes of relationship between the sizes of reverse micelles before and after extraction of peanut protein and their extraction rates
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摘要: 研究了在不同条件下萃取花生蛋白前后丁二酸二异辛酯磺酸钠(AOT)/异辛烷反胶束粒径变化及前萃率和后萃率变化,考察了反胶束粒径变化与前萃率和后萃率之间相互关系的变化规律。结果表明,KCl浓度增加,使粒径减小,前萃率也相应降低;在0.04~0.09 g/m L范围内,AOT浓度越大,空胶束及实胶束粒径越大,但是前萃率降低,而后萃率则相反;缓冲液p H对空胶束粒径影响不显著,但通过静电相互作用影响实胶束粒径和萃取率;不同的离子种类对反胶束粒径及花生蛋白的萃取影响差异显著,其中含Mg Cl2的缓冲溶液在添加量为0.03 m L/m L前萃率达到最大值98.55%±0.60%。反胶束粒径与萃取率之间的关系会受到许多因素的影响,不同因素影响下,二者之间的关系不同;并得出反胶束前萃花生蛋白的模型符合"水壳模型"。Abstract: This study evaluated the sizes of reverse micelles before and after the extraction of peanut protein by AOT/isooctane reverse micelles and investigated the relationship between the size change and extraction rates.The results showed that increasing the concentration of KCl,the particle sizes and forward extraction rates decreased. And increasing the concentration of AOT in the range of 0.04 ~0.09 g/m L,the empty reverse micelles and full reverse micelles increased,forward extraction rates increased,while the backward extraction rates were on the contrary. The p H had no significant influence on empty reverse micelles,but could influence full reverse micelles sizes and extraction rates by electrostatic interaction. The ion species had significant effects on the sizes of reverse micelles and extraction of peanut protein,when the addition amount of buffer solution containing Mg Cl2 was 0.03 m L/m L,the forward extraction rate reached the peak of 98.55%±0.60%. The relationship between reverse micelles sizes and extraction rates could influenced by many factors,and their relation varied under different conditions. The model of the forward extraction process was “water-shell model”.
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Keywords:
- reverse micelle /
- peanut protein /
- size /
- forward extraction rate /
- backward extraction rate
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