Isolation and characterization of fenvalerate degrading strain and optimization of degradation conditions
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摘要: 采用富集培养法从喷施拟除虫菊酯类农药的菜园土壤中,分离得到一株能降解氰戊菊酯的细菌BFE-023。经生理生化和16S r DNA序列分析,将菌株BFE-023鉴定为地衣芽孢杆菌（Bacillus licheniformis）。应用Plackett-Burman实验设计确定了影响该菌株降解氰戊菊酯的主要影响因素,利用响应面分析法优化了其降解条件。在优化条件下,研究菌株BFE-023对氰戊菊酯的降解过程及其中间产物3-苯氧基苯甲酸（3-PBA）的生成规律。结果表明,培养时间和降解体系中氰戊菊酯浓度及氯化铁含量是影响其降解的主要因素,优化条件下60 h内对氰戊菊酯降解率可达到88.71%,与所建立的模型预测值（88.78%）相吻合。菌株BEF-023降解氰戊菊酯的过程中,降解中间产物3-PBA的生成量呈现先明显增加后逐渐减少的趋势,说明菌株BEF-023可能具备继续降解中间产物3-PBA的能力。Abstract: Bacterial strain BFE-023 with high ability to degrade fenvalerate was isolated from garden soil which contaminated by pyrethroid pesticides. According to physiological and biochemical characteristics and 16 S r DNA sequence,the bacterial strain was identified as Bacillus licheniformis. The main factors influencing fenvalerate degradation were confirmed by using Plackett-Burman design,and the degradation condition was optimized with response surface method. Under the optimal conditions,the degradation of fenvalerate and the generation of 3- phenoxy benzoic acid( 3- PBA) were researched. The results showed that fenvalerate concentration,incubation time and ferric chloride content were the major factors which influencing fenvalerate degradation. In addition,the degradation rate of fenvalerate under the optimized conditions in 60 h reached88.71%,which was similar to predictive value from response surface model(88.78%). The concentration of 3-PBA in the degradation system of fenvalerate was increased obviously at first and then decreased gradually. It was proved that the strain BFE-023 could degrade the intermediate 3-PBA.
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