Using high-energy hybrid particle field mutation breeding high yield Monacolin K and low yield citrinin of Monascus strains
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摘要: 利用北京正负电子对撞机直线加速器E2束流打靶产生高能混合粒子场,以64.3 Gy的剂量处理两株紫色红曲霉（Monascus Purpureus）M1和M2,经初筛、复筛后采用HPLC法检测诱变菌株中Monacolin K含量,并进行遗传稳定性实验,从而选育高产Monacolin K、低产桔霉素的突变株。结果表明:经混合粒子场辐照后,紫色红曲霉M1和M2分别筛选出43株和22株正突变株,突变率分别为74.64%和56.72%,其中正突变率分别为60.56%和32.84%。突变株M1-20、M2-4发酵液中Monacolin K含量分别达到了421.69 mg/L和406.68 mg/L,较出发菌株分别提高了142.14%和101.27%,经5次传代培养后产Monacolin K的能力仅下降了4.21%和1.65%,并且其发酵液中桔霉素的含量均在0.010.04 mg/L之间,与出发菌株相比,桔霉素含量均明显下降。高能混合粒子场可引起紫色红曲霉菌落形态及色泽发生改变,突变率高,可获得遗传性能稳定的突变株,是一种可应用到微生物诱变育种的新方法。
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关键词:
- 紫色红曲霉 /
- Monacolin K /
- 混合粒子场 /
- 菌株选育
Abstract: Using of the Beijing electron-positron collider linear accelerator E2 beam shooting produce high-energy hybrid particle field processed two Monascus Purpureus strains M1 and M2 with the dose of 64.3 Gy,after initial and second screening,using HPLC method to detect the Monacolin K content in the mutant,and genetic stability experiments,thus breeding high Monacolin K and low yield citrinin mutant. The results showed that:after hybrid particle field irradiated,Monascus Purpureus M1 and M2 obtained 43 and 22 high yield Monacolin K mutants,respectively,the mutation rate was 74.64% and 56.72%,respectively,in which the positive mutation rate was 60.56% and 32.84%,respectively. Monacolin K content of M1-20 and M2-4 mutant reached421.69 mg/L and 406.68 mg/L,which improved 142.14% and 101.27% than the original strain,respectively,Monacolin K content of M1-20 and M2-4 mutant decreased 4.21% and 1.65% after subculture for 5 times,respectively,and citrinin content of two mutant were between 0.01 mg/L and 0.04 mg/L,which compared with the original strain,citrinin content were significantly decreased. High-energy hybrid particle field could cause colony morphology and color change of Monascus Purpureus,high mutation rate,the mutant strains of genetic stable performance could be obtained,it was a kind of new method that could be applied in the microbial mutation breeding.-
Keywords:
- Monascus Purpureus /
- Monacolin K /
- hybrid particle field /
- strain screening
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