Abstract: Using of the Beijing electron-positron collider linear accelerator E2 beam shooting produce high-energy hybrid particle field processed two Monascus Purpureus strains M1 and M2 with the dose of 64.3 Gy,after initial and second screening,using HPLC method to detect the Monacolin K content in the mutant,and genetic stability experiments,thus breeding high Monacolin K and low yield citrinin mutant. The results showed that:after hybrid particle field irradiated,Monascus Purpureus M1 and M2 obtained 43 and 22 high yield Monacolin K mutants,respectively,the mutation rate was 74.64% and 56.72%,respectively,in which the positive mutation rate was 60.56% and 32.84%,respectively. Monacolin K content of M1-20 and M2-4 mutant reached421.69 mg/L and 406.68 mg/L,which improved 142.14% and 101.27% than the original strain,respectively,Monacolin K content of M1-20 and M2-4 mutant decreased 4.21% and 1.65% after subculture for 5 times,respectively,and citrinin content of two mutant were between 0.01 mg/L and 0.04 mg/L,which compared with the original strain,citrinin content were significantly decreased. High-energy hybrid particle field could cause colony morphology and color change of Monascus Purpureus,high mutation rate,the mutant strains of genetic stable performance could be obtained,it was a kind of new method that could be applied in the microbial mutation breeding.