阿魏菇子实体多糖的结构及其抗氧化活性研究
Structural analysis and antioxidant activities of polysaccharides from the fruiting bodies of Pleurotus ferulae Lenzi
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摘要: 以从阿魏菇子实体中分离纯化得到的多糖-阿魏菇多糖（Pleurotus ferulae Lenzi polysaccharides,PFLPs）作为研究对象,对其化学结构、链构象以及抗氧化活性进行了研究。实验结果表明,PFLPs含有鼠李糖、木糖、甘露糖、葡萄糖和半乳糖五种单糖,摩尔比为1∶1.26∶0.85∶12.40∶4.31,并且糖链内具有β-D-葡聚糖结构。链构象分析表明PFLPs是一种具有三股螺旋结构的多糖。体外抗氧化活性实验表明,PFLPs具有较高的DPPH自由基、ABTS+自由基和羟自由基清除活性,适度的超氧阴离子自由基清除活性,还原力活性的和Fe2+的螯合活性。结果表明,PFLPs是一种很有潜力的天然抗氧化剂。Abstract: A polysaccharide fraction PFLPs was isolated and purified from the fruiting body of Pleurotus ferulae Lenzi. The chemical structure, chain conformation and antioxidant activities of PFLPs were investigated. The results indicated that PFLPs was mainly composed of Rhamnose,Xylose,Mannose,Glucose and Galactose with a molar ration of 1 ∶1.26 ∶0.85 ∶12.4 ∶4.31,and indicated the presence of β-D-glucan in the PFLPs. The chain conformation analysis showed that PFLPs was a triple helical polysaccharide. The antioxidant activity test in vitro revealed that PFLPs exhibited high DPPH radical,ABTS+radical and hydroxyl radical scavenging activities,moderate superoxide radical scavenging activities,reducing power and Fe~(2+) chelating activities. The results suggested that PFLPs could be used as a potential natural antioxidant.
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