采用FCF染色法测定粮食破碎率

任强; 姜平; 张来林; 李兴军

doi:10.13386/j.issn1002-0306.2016.02.001

采用FCF染色法测定粮食破碎率

1. 河南工业大学粮油食品学院
2. 国家粮食局科学研究院

基金项目:

粮食公益性行业科技专项(201313001-03)；

详细信息

作者简介:
任强(1990-),男,硕士研究生,研究方向:粮食储藏与食品工程,E-mail:renqiang1990@126.com。;

姜平(1987-),女,硕士,实习研究员,研究方向:粮食生化与多糖工程,E-mail:jingping2552@163.com。;

李兴军(1971-),男,博士,副研究员,研究方向:粮食生化与多糖工程,E-mail:lixingjun888@aliyun.com。;

中图分类号: TS210.7
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出版历程
- 收稿日期: 2015-03-18

Biochemical method for evaluation of grain damage with fast green FCF dye

College of Grain,Oil and Food,Henan University of Technology
Academy of the State Administration of Grains

摘要

摘要: 根据染色剂快绿FCF与粮粒破碎部分能够形成蓝色复合物,该复合物溶于稀Na OH溶液,本研究采用比色方法定量了OD₆₁₀与粮食破碎率之间的关系。建立了FCF染色法测定玉米、小麦及糙米破碎率方法,对5²0 g样品用体积为样品质量克数2倍的0.1%FCF染料染色20 min后,用蒸馏水冲洗直到水显示无色,再用体积为样品质量克数2倍的0.01 mol·L^-1Na OH溶液洗脱样品30 min,对洗脱液采用紫外分光光度计测定OD₆₁₀。整个操作在1 h内完成。该方法检测玉米、小麦及糙米破碎率范围分别在40%、20%及60%以上,但是不适合大米和大豆破碎率检测。OD₆₁₀（y）与破碎率（x）之间的线性方程对玉米、小麦及糙米代表品种分别为y=0.034x+0.316、y=0.071x+0.204、y=0.049x+0.206,相关系数高于0.998。
- 粮食破碎 /
- 快绿FCF /
- 小麦 /
- 玉米 /
- 糙米
Abstract: The complexes between Fast Green FCF dye and the damaged part of a grain were vivid,and were soluble in sodium hydroxide solution. The study established a biochemical method for evaluation of grain damage by FCF dye. Five to twenty grams of samples were soaked for 20 min by 10 to 40 m L of 0.1% FCF dye dissolved in 0.01 mol·L~(-1)Na OH solution,then the samples were rinsed off by distilled water till no blue color in washed water,finally the samples were bleached in 10 to 40 m L of 0.01 mol·L~(-1)Na OH for 30 min in order to recover the complexes formed between FCF dye and the damaged part of grain. The bleaching solution was used to determine absorbency in 610 nm. This protocol was carried out within 1 hour for determination of grain damage. The damaged rates for shelled corn,brown rice,and wheat were respectively detected by this method over the range of 40%,60% and 20%,but it can't be used to detect the breakage percent of milled rice and soybean. The linear equations of between OD_(610)(y) and sample breakage percent(x) for shelled corn,wheat,and brown rice were y=0.034x+0.316,y=0.071x+0.204,y=0.049x+0.206 and,respectively,with determined coefficient higher than 0.998.
- grain damage /
- fast green FCF /
- wheat /
- shelled corn /
- brown rice

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参考文献(15)

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