咖啡豆α-半乳糖苷酶的同源建模及分析

陶佳妮; 李赟; 姚娣; 沈汪洋; 陈轩; 周坚

doi:10.13386/j.issn1002-0306.2016.01.056

咖啡豆α-半乳糖苷酶的同源建模及分析

陶佳妮¹,
李赟¹,
姚娣¹,
沈汪洋^1,2,
陈轩¹,
周坚^1,2

武汉轻工大学食品科学与工程学院
农产品加工湖北省协同创新中心

基金项目:

国家自然科学基金青年科学基金项目(31301415)；

详细信息

作者简介:
陶佳妮(1991-),女,硕士,研究方向:食品生物化学,E-mail:18671272080@163.com。;

沈汪洋(1978-),男,博士,副教授,研究方向:食品生物化学,E-mail:whwangyangshen@126.com。;

中图分类号: TS273
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- 文章访问数: 162
- HTML全文浏览量: 20
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出版历程
- 收稿日期: 2015-05-14

Analysis and homology modeling of coffee- bean α- galactosidase

College of Food Science and Engineering,Wuhan polytechnic University
Hubei Collaborative Innovation Center for Processing of Agricultural Products

摘要

摘要: 为进一步了解咖啡豆α-半乳糖苷酶的结构,对咖啡豆α-半乳糖苷酶的理化性质、二级结构及三级结构等进行了理论分析和预测,并在三级结构的基础上进行同源建模研究。分析表明,该酶呈酸性,亲水,稳定。二级结构以α-螺旋和无规则卷曲为主,其空间结构与大米α-半乳糖苷酶相似度较高,以此为模版构建了可靠的三维结构模型。模型的一侧是由8个α-螺旋和8个β-转角形成的球状结构,另一侧是8个β-转角。实验结果为咖啡豆α-半乳糖苷酶的空间结构和催化活性位点的研究提供了理论依据。
- 咖啡豆α-半乳糖苷酶 /
- 蛋白质结构分析 /
- 同源建模
Abstract: The primary and spatial structures of coffee- bean α- galactosidase were studied to further determine its structure and function. The results showed that the enzyme protein was hydrophilic,acidic and stable. The secondary structure was dominated by α- helices and random coils. The three- dimensional structures of coffeebean α- galactosidase were constructed based on the template of rice α- galactosidase by homology modeling because their spatial structures were similarity.The side of the structure model was 8 α- helices and 8 β- turns,and the other was 8 β- turns. The experimental results provided the theoretical basis for the study on the spatial structure and catalytic site of coffee- bean α- galactosidase.
- coffee-bean α-galactosidase /
- protein structure analysis /
- homology modeling

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