Protective effects of Enteromorpha polysaccharides of Jiangsu province on the ethanol-induced gastric mucosal lesion in rats
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摘要: 为研究江苏地产浒苔多糖对酒精性大鼠胃黏膜损伤的保护作用和机制,将Wistar大鼠随机分成生理盐水组、模型组、浒苔多糖各剂量组（100,200,400 mg/kg）以及阳性对照组（奥美拉唑,300 mg/kg）,每组各10只。采用酒精灌胃致胃黏膜损伤模型。给予浒苔多糖7 d后,观察胃黏膜组织的大体和病理组织改变,测定胃液分泌量、胃酸浓度,胃组织中黏液蛋白、丙二醛（MDA）、氧化物歧化酶（SOD）、谷胱甘肽还原酶（GSH-Px）、大鼠白细胞介素1β（IL-1β）、肿瘤坏死因子（TNF-α）、以及环氧酶（COX-2）等生化指标的活力或含量。结果表明:浒苔多糖能明显改善胃黏膜组织损伤情况,降低损伤积分（p<0.01）,提高酒精性损伤抑制率;在高剂量组能显著降低胃液量的分泌（p<0.05）,提高胃组织GSH-Px,降低炎性因子IL-1β和TNF-α的释放（p<0.01）;各剂量组均能降低胃组织中MDA的含量和COX-2的活力（p<0.05或p<0.01）,显著提高SOD的活力以及胃黏液蛋白的浓度（p<0.05或p<0.01）。江苏地产浒苔多糖对大鼠急性酒精性胃黏膜损伤具有一定保护作用,其机制可能与增强胃黏膜保护因子,提高抗氧化、降低脂质过氧化、抑制炎症因子释放的能力有关。Abstract: In order to study the protective effects and mechanism of Enteromorpha polysaccharides( EP) of Jiangsu province on the ethanol-induced gastric mucosal lesion of rats in vivo,total 60 Wistar rats were randomly divided into EP groups(100,200,400 mg/kg),negative control group,model group,and positive control group(Omeprazole,300 mg/kg),i.e. 10 cases in each group. The model of acute gastric mucosa lesion was induced with absolute alcohol. After treated with EP with 7 days,the general and pathological integral of gastric mucosa lesion were observed. The levels of gastric juice volume,total titrable acidity,mucin,MDA,GSH-Px,IL-1β,TNF-α content,SOD and COX-2 activity were measured. Results :The gastric lesions were significantly reduced by all doses of EP(p <0.01). EP administration in high doses markedly reduced the volume of gastric juice( p < 0. 05), increased the GSH- Px tissue levels, and reduced the levels of pro-inflammatory markers IL- 1β and TNF- α( p < 0. 01). Moreover, all doses of EP significantly decreased the elevated MDA and COX-2 tissue levels(p<0.01 or p<0.05),whereas increased the SOD and mucin content(p <0.01 or p <0.05) in model rats. In a conclusion,these results suggested that EP could exert a beneficial gastroprotective effect through their anti-oxidant and anti-inflammatory properties.
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[1] 管华诗,王曙光.中华海洋本草[M].上海:上海科学技术出版社,2009. [2] Zhao J,Jiang P,Liu Z Y,et al.The Yellow Sea green tides were dominated by one species,Ulva(Enteromorpha)prolifera,from 2007 to 2011[J].Chinese Science Bulletin,2013,58(19):2298-2302.
[3] 徐大伦,黄晓春,杨文鸽,等.浒苔营养成分分析[J].浙江海洋学院学报:自然科学版,2003,22(4):318-320. [4] 于源,王鹏,李银平,等.浒苔多糖提取、结构与活性研究进展[J].中国渔业质量与标准,2013,3(3):83-88. [5] 齐晓辉,李红燕,郭守东,等.4种不同来源浒苔中多糖的提取分离及理化性质[J].中国海洋大学学报,2010,40(5):15-18. [6] 嵇国利,于广利,吴建东,等.爆发期条浒苔多糖的提取分离及其理化性质研究[J].中国海洋药物杂志,2009,28(3):7-12. [7] 吕海涛,肖宝石,高玉杰.浒苔多糖的酶法提取、纯化及初步结构鉴定[J].食品研究与开发,2013,43(8):33-35. [8] Kim J Y,Cho M L,Karnjanapratum S,et al.In vitro and in vivo immunomodulatory activity of sulfated polysaccharides from Enteromorpha prolifera[J].International Journal of Biological Macromolecules,2011,49(5):1051-1058.
[9] 许晶晶,唐志红,王景玉,等.浒苔多糖的纯化及抗氧化活性研究[J].食品工业科技,2009,30(10):134-136. [10] 林文庭,张智芳.浒苔多糖对荷瘤小鼠细胞抑制作用[J].中国公共卫生,2011,27(4):457-458. [11] 孙士红.碱提浒苔多糖降血脂作用研究[J].中国现代药物应用,2010,4(15):118-119. [12] 姚希贤.临床消化病学[M].天津:科学技术出版社,1995:489. [13] 梁桂宁,黄媛恒,梁志锋,等.紫菜多糖对急性酒精性胃黏膜损伤小鼠胃组织PGE2及NO的影响[J].甘肃中医学院学报,2012,29(2):7-8. [14] 梁桂宁,黄媛恒,贾晓栋,等.紫菜多糖对急性酒精灌胃小鼠胃组织SOD,GSH-Px的影响[J].中国实用医药,2012,7(4):248-249. [15] 阮研硕,赵江燕,李艳梅,等.岩藻多糖对急性酒精性胃黏膜损伤的保护作用[J].中国食品学报,2015,15(1):19-24. [16] 王明莹.肠浒苔碱提水提多糖性质的比较[D].长春:东北师范大学,2008. [17] Dubois M,Gilles K A,Hamilton J K,et al.Colorimetric method for determination of sugars and related substances[J].Analytical Chemistry,1956,28(3):350-366.
[18] Brown R E,Jarvis K L,Hyland K J.Protein measurement using bicinchoninic acid:elimination of interfering substances[J].Analytical Chemistry,1989,180(1):136-139.
[19] Bitter T,Muir H M.A modified uronic acid carbazole reaction[J].Analytical Biochemistry,1962,4:330-334.
[20] Therho T T,Hartiala K.Method for determination of the sulfate content of glycosamino glycans[J].Analytical Biochemistry,1971,41:471-476.
[21] 中华人民共和国卫生部国家食品药品监督管理局.国食药监保化[2012]107号关于印发抗氧化功能评价方法等9个保健功能评价方法的通知[S].http://www.sda.gov.cn/WS01/CL0847/71257.html,2012. -
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