桂花酶解物中酚类物质及其抗氧化活性研究

李红领; 李春阳; 曾晓雄; 刘晓林

doi:10.13386/j.issn1002-0306.2015.24.009

桂花酶解物中酚类物质及其抗氧化活性研究

南京农业大学食品科技学院
江苏省农业科学院农产品加工所
徐州林泉绿色食品饮料厂

基金项目:

江苏省农业科技自主创新资金(CX(14)2120)；江苏省苏北科技专项资金(富民强县)项目(BN2014088)； 2014年中央财政农业技术推广资金项目(TG(14)113)；

详细信息

作者简介:
李红领(1990-),男,硕士研究生,研究方向:功能性食品,E-mail:2013108070@njau.edu.cn。;

李春阳(1966-),男,研究员,研究方向:营养与活性物质,农产品精深加工,E-mail:lichunyang968@126.com。;

中图分类号: TS201.4
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出版历程
- 收稿日期: 2015-04-19

Study on the phenolics of hydrolysates from Osmanthus fragranslour and its antioxidant activity

College of Food Science and Technology,Nanjing Agriculture University
Institute of Farm Product Processing,Jiangsu Academy of Agriculture Science
Beverage Factory of Organic Foods in Linquan

摘要

摘要: 运用LC-MS/MS对桂花酶解物中的酚类物质进行研究,发现桂花酶解物中含有36种酚类物质,包括单咖啡酰基奎宁酸、咖啡酸4-O-葡萄糖苷、5-O-对香豆酰基奎宁酸、4-O-对香豆酰基奎宁酸、木犀草素-7-O-6″-丙二酰基葡萄糖苷、麦角甾苷、异麦角甾苷等物质。抗氧化活性研究发现,桂花酶解物浓度大于0.8 mg/m L时,对ABTS+、DPPH·自由基清除率达90%以上,均显著高于芦丁（p<0.05）,略低于Trolox;桂花酶解物的还原力为3.0左右,显著高于芦丁的1.0左右（p<0.05）,稍高于Trolox;桂花酶解物的TEAC和ORAC值分别为648.66μmol Trolox/g和813.53μmol Trolox/g,均显著高于芦丁（p<0.05）;桂花酶解物的ABTS+、DPPH·自由基清除能力和还原力与总酚含量均呈显著的线性正相关。
- 桂花酶解物 /
- LC-MS/MS /
- 抗氧化活性 /
- 总酚
Abstract: The present study investigated the phenolics of hydrolysates of Osmanthus fragranslour by LC-MS/MS. The research results demonstrated that hydrolysates of Osmanthus fragranslour contained 36 varities of phenolics,including Caffeyolquinic acid,Caffeic acid 4-glucoside 4-O-p-coumaroylquinic acid,5-O-p-coumaroylquinic acid,Luteolin-7-O-6″-malonylglucoside,Acteoside,Isoacteoside etc. This study also evaluated the antioxidant activity of hydrolysates of Osmanthus fragranslour,Trolox and Rutin. It showed that scavenging activity of ABTS+and DPPH· free radical and reducing power of hydrolysates from Osmanthus fragranslour reached 90 % and were slightly lower than Trolox, but significantly higher than Rutin( p < 0. 05), when the concentration was greater than 0.8 mg/m L. The values of TEAC and ORAC of hydrolysates from Osmanthus fragranslour were 648.66 μmol Trolox/g and 813.53 μmol Trolox/g and significantly higher than Rutin(p<0.05). It was observed that the scavenging activity of ABTS+and DPPH· free radical and reducing power of hydrolysates from Osmanthus fragranslour had a significantly positive correlation with the contents of polyphenolics.
- hydrolysates from Osmanthus fragranslour /
- LC-MS/MS /
- antioxidant activity /
- total polyphenolics

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参考文献(18)

[1]	田成.桂花果实多酚的超声波提取及抗氧化活性研究[J].食品科学,2012,32(24):106-110.
[2]	靳熙茜,汪海波.桂花总黄酮提取及其体外抗氧化性能研究[J].粮食与油脂,2009(11):42-45.
[3]	施余杰.桂花有效成分体外α-葡萄糖苷酶活性及药动因素研究[D].开封:河南大学,2013.
[4]	岳淑梅,王金梅,康文艺.桂花三萜类成分及降血脂作用的研究[J].中国实验方剂学杂志,2013,19(24):126-128.
[5]	吴如娥.桂花萃取物对游乐区场所劳工抗氧化能力提升对听损及疲劳症候减少之研究[D].台湾:中华医事科技大学,2011.
[6]	李春阳,冯进.蓝莓叶多酚与蓝莓果渣多酚提取物抗氧化活性研究[J].食品工业科技,2013(7):56-60.
[7]	冯进,李敏,曾晓雄,等.大孔树脂纯化蓝莓叶多酚及其组成分析[J].食品科学,2013,34(10):86-91.
[8]	Bhattacherjee A,Ghosh T,Sil R,et al.Isolation and characterisation of methanol-soluble fraction of Alternanthera philoxeroides(Mart.)-evaluation of their antioxidant,α-glucosidase inhibitory and antimicrobial activity in in vitro systems[J].Natural product research,2014,28(23):2199-2202.
[9]	Molyneux P.The use of the stable free radical diphenylpicrylhydrazyl(DPPH)for estimating antioxidant activity[J].Songklanakarin J Sci Technol,2004,26(2):211-219.
[10]	黄仁术,易凡,何惠利,等.金荞麦(-)-表儿茶素抗氧化活性研究[J].食品科学,2014(15):26.
[11]	Thaipong K,Boonprakob U,Crosby K,et al.Comparison of ABTS,DPPH,FRAP,and ORAC assays for estimating antioxidant activity from guava fruit extracts[J].Journal of Food Composition and Analysis,2006,19(6):669-675.
[12]	Kevers C,Sipel A,Pincemail J,et al.Antioxidant Capacity of Hydrophilic Food Matrices:Optimization and Validation of ORAC Assay[J].Food Analytical Methods,2014,7(2):409-416.
[13]	Yao F,Huang Z,Li D,et al.Phenolic components,antioxidant enzyme activities and anatomic structure of longan fruit pericarp following treatment with adenylate triphosphate[J].Scientia Horticulturae,2014,180:6-13.
[14]	Kis E,Rajnai Z,Ioja E,et al.Mouse Bsep ATPase assay:a nonradioactive tool for assessment of the cholestatic potential of drugs[J].Journal of Biomolecular Screening,2008,14(1):10-15.
[15]	Khan R A,Khan M R,Sahreen S,et al.Assessment of flavonoids contents and in vitro antioxidant activity of Launaea procumbens[J].Chem Central J,2012,6(1):43.
[16]	Lin L Z,Harnly J M.Identification of the phenolic components of chrysanthemum flower(Chrysanthemum morifolium Ramat)[J].Food Chemistry,2010,120(1):319-326.
[17]	Zhang W B,Wang Z C,Zhang L Y.Determination of 10caffeoylquinic acids and 22 flavonoids in chrysanthemum samples by ultra-high performance liquid chromatography-diode array detection-tandem mass spectrometry[J].Chinese Journal of Analytical Chemistry,2013(12):14.
[18]	Clifford M N.Hierarchical scheme for LC-MSn identification of chlorogenic acids[J].J Agric Food Chem,2003,51(10):2900-2911.