恒温超声辅助提取诃子FRAP类抗氧化活性物质的研究

刘梦莹; 江慎华; 曲文娟; 金洪光; 廖亮; 马海乐; 沈勇根; 徐明生

doi:10.13386/j.issn1002-0306.2015.20.042

恒温超声辅助提取诃子FRAP类抗氧化活性物质的研究

刘梦莹¹,
江慎华^1,2,3,
曲文娟^1,2,
金洪光¹,
廖亮¹,
马海乐^1,2,
沈勇根^1,3,
徐明生^1,3

1. 九江学院药学与生命科学学院
2. 江苏大学食品与生物工程学院江苏省农产品物理加工重点实验室
3. 江西农业大学食品科学与工程学院江西省天然产物与功能食品重点实验室

基金项目:

江苏省青年自然科学基金(BK2012287)；国家自然科学基金(31360371)；江西省自然科学基金(20132BAB204030)；江西省科技支撑计划(20123BBF60150)；江苏省农产品物理加工重点实验室开放课题(JAPP2010-5)；江西省高等学校大学生创新创业计划项目；江西省天然产物与功能食品重点实验室开放基金；九江市科技支撑计划(201438)；

详细信息

作者简介:
刘梦莹(1993-),女,大学本科,主要从事天然产物研究与开发方面的工作,E-mail:1048188096@qq.com。;

江慎华(1973-),男,博士,副教授,主要从事天然产物研究与开发方面的工作,E-mail:jiangshenhua66@163.com。;

中图分类号: TS202.3
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出版历程
- 收稿日期: 2015-03-08

The FRAP antioxidants extraction from Terminalia chebula Retz based on ultrasound assisted extraction with constant temperature

School of Pharmacy and Life Science,Jiujiang University
School of Food and Biological Engineering,Jiangsu Provincal Key Lab of Physical Processing of Agricultural Products,Jiangsu University
College of Food Science and Engineering,Jiangxi Key Laboratory of Natural Products and Functional Foods,Jiangxi Agricultural University

摘要

摘要: 为了探索恒温超声辅助提取诃子FRAP类抗氧化活性物质提取效果,本文采用该技术首先对提取工艺进行优化,进而通过比较实验和扫描电镜分析该技术提取高效的原因。结果表明,诃子FRAP类抗氧化活性物质恒温超声辅助提取最佳工艺条件为:采用60%乙醇、超声功率180 W、超声频率80 k Hz、超声温度70℃;比较分析发现,该工艺提取效率显著高于水浴振荡提取工艺(p<0.001);扫描电镜分析发现,恒温超声辅助提取对诃子原料细胞结构破坏更严重,从而增强了提取效率。恒温超声辅助提取技术对诃子抗氧化活性物质提取效率高,本文可为诃子产业化开发提供依据。
- 恒温超声辅助提取 /
- 诃子 /
- 抗氧化活性物质 /
- 扫描电镜
Abstract: In order to explore the extracting effect of antioxidants from Terminalia chebula Retz(TCR) based on ultrasound assisted extraction with constant temperature technology(UAECTT),at first,the extraction technology of antioxidants were optimized,then the reasons for high efficiency of this extraction technology were analysized by comparative experiments and scanning electron microscope(SEM). The optimal parameters of the extracting technology were as follows the concentration of ethanol 60%,the ultrasound frequency 80 k Hz,the ultrasound power 180 W,and the extracting temperature 70 ℃. The extraction efficiency of UAECTT dramaticlly was higher than that of water bath oscillator extraction(WBOE)(p<0.001) based on the comparative analysis. A comparison of SEM images of TCR powders indicated the extraction efficiency was higher treated by UAECTT with the more serious disruption of cell structure and the more sufficient interaction. The technology of UAECTT was proved as one technology with high efficiency for extracting TCR antioxidants and this study provided the basis for future industrializing development of TCR.
- ultrasound assisted extraction with constant temperature /
- Terminalia chebula Retz /
- antioxidants /
- scanning electron microscope

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