三峡库区乌皮香核桃隔膜皂苷提取及抗氧化测定

基金项目:

重庆市自然科学基金项目(CSTC2009BB5236)； 12科研创新团队建设三峡库区农产品绿色保鲜技术； 2014年重庆三峡学院科学研究青年资助项目(14QN26)；

详细信息

Antioxidant activity of ultrasonic extraction isolates from three gorges reservoir area Juglans regia of Wu walnut

Biotechnology and Food Basic Experimental Teaching Center(Municipal Experimental Teaching Demonstration Center)College of Life Science and Engineering,Chongqing Three Gorges University

摘要: 本实验采用超声波法,响应面设计提取三峡库区特产核桃隔膜皂苷。结果显示核桃隔膜中皂苷提取的最佳提取工艺参数为:提取功率300 W,提取时间为96 s,料液比为1∶33(g/m L),皂苷的提取得率为5.98%。通过DPPH,ABTS法对核桃隔膜皂苷提取物进行抗氧化实验,表明皂苷提取物对自由基清除效果明显,有较强的抗氧化活性。
- 核桃隔膜 /
- 皂苷 /
- 超声波提取 /
- 响应面分析 /
- 抗氧化性
Abstract: The total saponins were examined in the dissepiment of Wu walnut. On the basis of single factor experiments with the method of ultrasonic-assisted extraction,optimal parameters of extraction process was researched by using response surface method. A mathematical model including material/liquid ratio,ultrasonic treatment time and extraction temperature was created. By typicality analysis,the optimum process conditions were as follows:the ultrasonic treatment time was 96 s,the ultrasonic power was 300 W and the material/liquid ratio was 1∶33(g/m L). Under such conditions,an optimal total saponins yield of 5.98% was obtained. Saponins displayed strong antioxidant effect and scavenging capacity against DPPH,ABTS radical,and more strengthen with the increase in the concentration of total crude saponins extract.
- Juglans regia /
- saponins /
- ultrasonic extraction /
- response surface method /
- antioxidant