藏灵菇发酵产胞外多糖的影响因素及其应用研究

王辑; 田政; 赵笑; 曹永强; 杨贞耐

doi:10.13386/j.issn1002-0306.2015.18.032

藏灵菇发酵产胞外多糖的影响因素及其应用研究

1. 北京工商大学北京市食品添加剂工程技术研究中心
2. 北京顺鑫农业股份有限公司

基金项目:

北京市教委科研计划重点项目(KZ201310011011)；国家自然科学基金面上项目(31371804)；

详细信息

作者简介:
王辑(1986-),男,博士研究生,研究方向:乳品加工,E-mail:wangji198644@163.com。;

杨贞耐(1965-),男,博士,教授,研究方向:乳品加工及交叉学科的理论和应用,E-mail:yangzhennai@th.btbu.edu.cn。;

中图分类号: TS219;TQ281
计量
- 文章访问数: 170
- HTML全文浏览量: 30
- PDF下载量: 193
出版历程
- 收稿日期: 2014-10-30

Study on the factors affecting exopolysaccharide production by fermentation with Tibetan Kefir and its application

Beijing Engineering and Technology Research Center of Food Additives,Beijing Technology and Business University
Beijing Shunxin Agriculture Co.,Ltd.

摘要

摘要: 利用苯酚-硫酸法从15个不同地区藏灵菇样品中筛选出3个高产胞外多糖样品（K4、K7、K12）。通过单因素分析确定K4、K7、K12高产胞外多糖的条件为:接种量K4为9%、K7和K12为7%;发酵时间K12为12 h、K4和K7为18 h;发酵温度均为37℃;脱脂乳浓度均为11%。藏灵菇K4、K12分别与商业发酵剂搭配制备发酵乳,结果表明,K12发酵乳的凝乳时间最短（4.52 h）,胞外多糖含量和活菌数分别保持在50 mg/L左右和8.20 log cfu/m L以上,且具有较高的持水力和黏度。
- 藏灵菇 /
- 胞外多糖 /
- 发酵乳 /
- 乳酸菌
Abstract: Exopolysaccharides(EPSs) production by 15 Tibetan Kefir samples collected from different regions were measured by phenol-sulfuric acid method,and three of them(K4,K7 and K12) with high EPS yields were selected. By single factor test,the fermentation conditions were obtained as follows:inoculation rate of 7% for K7 and K12,9% for K4,fermentation time of 12 h for K12,18 h for K4 and K7,fermentation temperature of37 ℃,skim milk concentration of 11%. The use of the Kefir samples K4 or K12 together with a commercial starter as a mixed starter in fermentation of whole milk showed that the combination of K12 and commercial starter gave the shortest coagulating time(4.52 h). During the 21 d of storage at 4 ℃,the fermented milk produced from this starter combination contained EPS of about 50 mg/L and viable bacterial counts of over8.20 log cfu/m L,maintaining higher water holding capacity and viscosity.
- Tibetan Kefir /
- exopolysaccharides /
- fermented milk /
- lactic acid bacteria