ε-聚赖氨酸产生菌的复合诱变筛选及其发酵培养基优化

吴晨奇; 扶教龙; 陈冬铨; 钱大伟; 李良智; 胡翠英; 王桃云

doi:10.13386/j.issn1002-0306.2015.18.029

ε-聚赖氨酸产生菌的复合诱变筛选及其发酵培养基优化

苏州科技学院化学生物与材料工程学院
苏州市新能源与低碳技术重点实验室

基金项目:

苏州市科技计划项目(SYN201317)；苏州科技学院科研基金项目(XKZ201411)；

详细信息

作者简介:
吴晨奇(1989-),男,硕士研究生,研究方向:生物化工、食品微生物,E-mail:612324wcq@163.com。;

扶教龙(1969-),男,副教授,主要从事生物化工、发酵工程方面的研究,E-mail:jlfu999@126.com。;

中图分类号: TQ920.1;TS201.3
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出版历程
- 收稿日期: 2014-12-21

Breeding of ε-poly-L-lysine producing strain with combined mutation and the optimization of its fermentation medium

School of Chemistry Biology and Material Engineering,Suzhou University of Science and Technology
Key Laboratory of New Energy and Low-carbon Technology of Suzhou City

摘要

摘要: 以白色链霉菌FQ-9为出发菌株,首次利用（甘氨酸+L-赖氨酸+磺胺胍）复合抗性,进行"紫外-氯化锂"复合诱变,筛选出一株ε-PL产量达（0.668±0.0045）g/L的突变菌株FQC-23,产量提高了15.37%,且其遗传性稳定。然后在单因素实验的基础上,应用响应面分析方法,对其发酵培养基进行优化,最终确定最佳培养基为（g/L）:葡萄糖46.1、酵母粉13.0、（NH4）2SO45.9、Mg SO4·7H2O 0.5、K2HPO40.8、KH2PO41.36、Fe SO4·7H2O 0.03、Zn SO4·7H2O 0.04,在此条件下,FQC-23的ε-PL产量达（1.090±0.0041）g/L,比出发菌株的产量提高了87.56%。
- ε-聚赖氨酸 /
- 抗性平板 /
- 诱变 /
- 响应面分析方法 /
- 白色链霉菌
Abstract: Using Streptomyces albulus FQ-9 as the initial strain mutated by UV combined with Li Cl,and using glycine +sulfaguanidine(SG) +L-lysine as selective markers. Then,a ε-PL-producing strain,Streptomyces albulus FQC-23 of the yield improved to(0.668±0.0045) g/L was screened,which was 15.37% higher than that of FQ-9. Moreover,based on single factor experiment,the fermentation medium of ε-PL producing strain FQC-23 were optimized by response surface methodology,and finally determined the optimal fermentation medium as follows(g/L):glucose 46.1,yeast powder 13.0,(NH4)2SO45.9,Mg SO4·7H2O 0.5,K2HPO40.8,KH2PO41.36,Fe SO4·7H2O 0.03,Zn SO4·7H2O 0.04. Under the optimized medium,the yield of ε-PL reached(1.090±0.0041) g/L and increased by 87.56% compared with the previous condition.
- ε-poly-L-lysine /
- resistant medium plate /
- mutation /
- response surface methodology /
- Streptomyces albulus