Simultaneous determination of four sulfonamide in animal derived food samples by polyvinylidene fluoride membrane-based dot immunoassay
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摘要: 建立了一种简便、快速、可靠的膜免疫芯片检测牛奶和猪肝样品中磺胺类药物总量的方法。以PVDF膜作为固相基质,通过优化包被抗原浓度和抗体的工作浓度,研究出了检测磺胺药物膜免疫分析法。结果表明,该法检测牛奶和猪肝样品中四种磺胺药物（磺胺嘧啶、磺胺甲基嘧啶、磺胺二甲嘧啶和磺胺对甲氧嘧啶）总量的检测阈值为50μg/kg,可以用于磺胺药物总量的筛查。此外,分别采用本方法、Ic-ELISA和HPLC测定了市场上随机购买的20份牛奶和8份猪肝样品,三者结果基本一致。本法结果可靠、简便直观、无需特殊的检测仪器,适合于在线监测和收购现场的快速检测。
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关键词:
- 聚偏氟乙烯膜 /
- 磺胺类药物 /
- 膜免疫检测 /
- 动物样品 /
- 间接竞争酶联免疫检测
Abstract: The development of polyvinylidene fluoride( PVDF) membrane- based dot- immunoassay for convenience and rapid detection of sulfonamides in milk and pork liver samples was described. Based on the PVDF membrane,a membrane-based dot immunoassay was to detect sulphonamides,and the influence of envelope antigen concentration and antibody concentration factors on the immunoassay was studied. Using this method,the cut-off level of the sum of sulfadiazine(SDZ),sulfamerazine(SMR),sulfadimidine(SM2),and sulfametoxydiazine(SMDZ) were 50 μg/kg in milk and pork liver. The total concentration of SDZ,SMR,SM2 and SMDZ were determined in 20 milk and in 8 pork livers samples by both the PVDF membrane-based immunoassay and Ic-ELISA,and the results showed no significant difference with the HPLC. These results suggested that this method could be a useful on-site screening tool for the rapid detection of sulphonamides(SDZ,SMR,SM2 and SMD) in milk and pork liver samples without special instrumentation.
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