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中国精品科技期刊2020

响应面法优化萌发菌HL-003胞内多糖提取工艺

郑朋朋, 李珊, 陈玉惠, 敖新宇

郑朋朋, 李珊, 陈玉惠, 敖新宇. 响应面法优化萌发菌HL-003胞内多糖提取工艺[J]. 食品工业科技, 2015, (15): 219-223. DOI: 10.13386/j.issn1002-0306.2015.15.037
引用本文: 郑朋朋, 李珊, 陈玉惠, 敖新宇. 响应面法优化萌发菌HL-003胞内多糖提取工艺[J]. 食品工业科技, 2015, (15): 219-223. DOI: 10.13386/j.issn1002-0306.2015.15.037
ZHENG Peng-peng, LI Shan, CHEN Yu-hui, AO Xin-yu. Optimization of extraction conditions of intracellular polysaccharides from HL-003 using response surface methodology[J]. Science and Technology of Food Industry, 2015, (15): 219-223. DOI: 10.13386/j.issn1002-0306.2015.15.037
Citation: ZHENG Peng-peng, LI Shan, CHEN Yu-hui, AO Xin-yu. Optimization of extraction conditions of intracellular polysaccharides from HL-003 using response surface methodology[J]. Science and Technology of Food Industry, 2015, (15): 219-223. DOI: 10.13386/j.issn1002-0306.2015.15.037

响应面法优化萌发菌HL-003胞内多糖提取工艺

基金项目: 

云南省优势特色重点学科生物学一级学科建设项目(50097505);

详细信息
    作者简介:

    郑朋朋(1990-),男,在读研究生,主要从事生物化学与分子生物学研究,E-mail:1104663437@qq.com。;

    敖新宇(1978-),男,硕士,副教授,主要从事分子生物学研究,E-mail:54700875@qq.com。;

  • 中图分类号: TQ281

Optimization of extraction conditions of intracellular polysaccharides from HL-003 using response surface methodology

  • 摘要: 为确定萌发菌HL-003胞内多糖提取工艺的最佳条件,以萌发菌HL-003胞内多糖提取得率为考察指标,在单因素实验的基础上,采用响应面法对主要提取工艺参数进行优化,并得到回归模型。结果表明:萌发菌HL-003胞内多糖提取工艺的最优条件参数为超声波处理时间19 min、液料比42 m L/g、浸提温度72℃、提取时间2.2 h,最大提取得率达2.03%。实验证明模型拟合程度良好,误差较小。 
    Abstract: Response surface methodology was used to optimize main process parameters for the extraction of polysaccharides from HL-003's intracellular broth based on polysaccharide extraction rate of one- factor tests.A regression model equation was fitted.The optimum extraction conditions were found to be ultrasound time 19 min,liquid- solid ratio 42 m L / g,extraction temperature 72 ℃ and extraction time 2.2 h.The extraction rate of polysaccharides under these conditions was up to 2.03%.The fitted regression model had excellent goodness of fit and showed lower prediction error.
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  • 期刊类型引用(1)

    1. 陈洋,王悦力,陈诗奇,李楠鑫,张伟,舒刚,徐傅能,李昊欢,林居纯,符华林. 基于寡肽转运蛋白的氟苯尼考肠吸收特性研究. 畜牧兽医学报. 2023(03): 1240-1248 . 百度学术

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  • 收稿日期:  2014-11-12

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