响应面法优化萌发菌HL-003胞内多糖提取工艺

郑朋朋; 李珊; 陈玉惠; 敖新宇

doi:10.13386/j.issn1002-0306.2015.15.037

响应面法优化萌发菌HL-003胞内多糖提取工艺

西南林业大学生命科学学院

基金项目:

云南省优势特色重点学科生物学一级学科建设项目(50097505)；

详细信息

作者简介:
郑朋朋(1990-),男,在读研究生,主要从事生物化学与分子生物学研究,E-mail:1104663437@qq.com。;

敖新宇(1978-),男,硕士,副教授,主要从事分子生物学研究,E-mail:54700875@qq.com。;

中图分类号: TQ281
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出版历程
- 收稿日期: 2014-11-12

Optimization of extraction conditions of intracellular polysaccharides from HL-003 using response surface methodology

Life Science College,Southwest Forestry University

摘要

摘要: 为确定萌发菌HL-003胞内多糖提取工艺的最佳条件,以萌发菌HL-003胞内多糖提取得率为考察指标,在单因素实验的基础上,采用响应面法对主要提取工艺参数进行优化,并得到回归模型。结果表明:萌发菌HL-003胞内多糖提取工艺的最优条件参数为超声波处理时间19 min、液料比42 m L/g、浸提温度72℃、提取时间2.2 h,最大提取得率达2.03%。实验证明模型拟合程度良好,误差较小。
- 胞内多糖 /
- 超声波 /
- 响应面优化 /
- 提取工艺
Abstract: Response surface methodology was used to optimize main process parameters for the extraction of polysaccharides from HL-003's intracellular broth based on polysaccharide extraction rate of one- factor tests.A regression model equation was fitted.The optimum extraction conditions were found to be ultrasound time 19 min,liquid- solid ratio 42 m L / g,extraction temperature 72 ℃ and extraction time 2.2 h.The extraction rate of polysaccharides under these conditions was up to 2.03%.The fitted regression model had excellent goodness of fit and showed lower prediction error.
- intracellular polysaccharides /
- ultrasound wave /
- response surface methodology /
- extraction technology

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