Abstract: A quick method was established for the determination of 57 pesticide residues in canned mushrooms by by gas chromatography with mass spectrometry(GC-MS) after modified dispersive solid-phase extraction(d-SPE). Graphene as d-SPE sorbent firstly was applied on the determination of 57 pesticide residues in canned mushrooms. Compared with previous primary-secondary amine(PSA) and octadecylsilance(ODS),graphene had advantage of effective impurity removal and low cost. The experiment was quantitative using external standard method and showed a good linearity over the ranger of 0.987~0.9998. The recoveries of most pesticides were from 70.2% to 130% except Fipronil and precision(n=5) were less than 10%,and the detection limit of 57 pesticides were from 0.8~19.7μg/kg. The result indicated that the developed method was suitable for the determination of 57 pesticide residues in canned mushrooms.