响应面法优化水黄芹总黄酮提取工艺及其抗氧化研究

甘秀海; 梁志远; 代雄; 赵超; 周欣

doi:10.13386/j.issn1002-0306.2015.13.042

响应面法优化水黄芹总黄酮提取工艺及其抗氧化研究

1. 贵州师范学院化学与生命科学学院
2. 贵州师范大学天然药物质量控制研究中心

基金项目:

贵州省应用化学特色重点学科(黔教科研发[2012]442号)；贵州省教育厅特色重点实验室项目(黔科合KY[2012]005号)；贵州师范学院科学研究项目(201209)；

详细信息

作者简介:
甘秀海 (1976-) , 男, 硕士, 副教授, 从事天然药物化学研究。;

周欣 (1962-) , 女, 博士, 教授, 研究方向:中药质量控制及新药开发。;

中图分类号: TQ28
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出版历程
- 收稿日期: 2014-10-27

Optimization of extracting total flavonoids from Phyla nodiflora ( L.) by response surface methodology and its antioxidant activity

The School of Chemistry and Life Science, Guizhou Normal College
The Research Center for Quality Control of Natural Medicine, Guizhou Normal University

摘要

摘要: 目的:确定水黄芹中总黄酮的最佳提取工艺,并评价其抗氧化活性。方法:在单因素实验基础上,利用响应面法优化水黄芹总黄酮的提取条件;通过总黄酮对DPPH·和ABTS·的清除作用来研究其抗氧化活性。结果:水黄芹总黄酮最佳提取工艺为乙醇浓度74%、料液比1∶30(g/m L)、提取温度72℃、提取时间115min,此条件下,总黄酮得率为1.59%,与预测值1.60%接近,其偏差为0.63%。水黄芹总黄酮对DPPH·和ABTS·具有较好的清除能力,其IC50值分别为7.67、12.88μg/m L。
- 水黄芹 /
- 总黄酮 /
- 响应面法 /
- 抗氧化
Abstract: Objective: To explore the optimum extraction process of total flavonoids from Phyla nodiflora ( L.) and evaluate the antioxidant activity. Methods: Based on the single- factor tests, the extraction conditions of total flavonoids from Phyla nodiflora ( L.) were optimized by response surface methodology. Meanwhile, the antioxidant activity of total flavonoids was assessed by scavenging assays of DPPH· and ABTS· Results: The optimum extraction conditions were as follows: ethanol concentration 74%, liquid- solid ratio 1 ∶ 30, extraction temperature72℃ and extraction time 115 min. Under the optimal conditions, the extraction rate of total flavonoids was 1.59%, which was close to the predicted value 1.60% with 0.63% bias.In addition, DPPH· and ABTS· radical scavenging capacity of total flavonoids was better, and IC50 of it was 7.67μg / m L and 12.88μg / m L, respectively.
- Phyla nodiflora (L.) /
- total flavonoids /
- response surface analysis /
- antioxidant

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参考文献(15)

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