酶法结合超声波提取粉葛葛根素的研究

李甜甜; 傅舒; 刘晓风; 李东; 王舒雅; 郑涛

doi:10.13386/j.issn1002-0306.2015.13.028

酶法结合超声波提取粉葛葛根素的研究

1. 南京工业大学
2. 中国科学院成都生物研究所
3. 中国科学院大学

基金项目:

中国科学院环境与应用微生物重点实验室开放基金项目(KLCAS-2013-05)；江苏省自然科学基金(BK20130932)；江苏省高校自然科学基金(13KJB530009)资助；

详细信息

作者简介:
李甜甜 (1989-) , 女, 硕士, 研究方向:天然产物提取。;

郑涛 (1982-) , 男, 博士, 研究方向:生物质能源化利用、生物电化学合成、天然产物绿色提取技术等。;

中图分类号: TQ464
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出版历程
- 收稿日期: 2014-08-20

Research of enzymatic hydrolysis assisted ultrasonic for extraction of puerarin from Kudzu

Collage of Biotechnology and Pharmaceutical, Nanjing Tech University
Chengdu Institute of Biology, Academy of Sciences
University of Chinese Academy of Sciences

摘要

摘要: 目的:以提高粉葛葛根素的得率为目的。方法:在优选出纤维素酶结合超声波的方法基础上,采用单因素实验和响应面实验法,对影响酶解反应的主要因素进行了优化。结果:最佳酶解反应条件为固液比1∶31(g/m L)、酶解时间9.8h、酶添加量31.84U/g,超声条件为功率150W、频率40k Hz、时间30min。在此条件下得率为0.728%,比优化前提高15.37%,比单纯超声波提取法和传统乙醇浸提法分别高出24.7%和28.0%。结论:初步建立了纤维素酶结合超声波技术提取粉葛葛根素的最优工艺条件。
- 纤维素酶 /
- 粉葛 /
- 葛根素 /
- 因素 /
- 响应面分析
Abstract: To increase yield of puerarin, enzymatic hydrolysis assisted ultrasonic extraction was used to extract puerarin from Kudzu. A Box- Benhnken design ( BBD) involving three main variables such as solid- liquid ratio, enzymatic time and enzyme dosage at three levels was employed based on single factor experiments.The optimal values of these variables were determined by response surface analysis. The optimal conditions were as follows:solid- liquid ratio of 1∶31 ( g / m L) , enzymatic time of 9.8h and enzyme dosage of 31.84 U / g.Moreover, the validity of the established model was verified.The experimental yield of puerarin of 0.728% was in good agreement with the predicted one of 0.723%, which was 15.37% higher than unoptimized group and separately 24.7% and 28.0%higher than ultrasonic extraction and ethanol extraction methods.
- cellulase /
- Kudzu /
- Puerarin /
- factors /
- response surface analysis

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