茶多酚对高尿酸血症小鼠尿酸水平的影响及机制研究

谭明亮; 陈刚

doi:10.13386/j.issn1002-0306.2015.12.066

茶多酚对高尿酸血症小鼠尿酸水平的影响及机制研究

谭明亮^1,2,
陈刚^1,2,3

1. 重庆工商大学天然药物研究重点实验室
2. 重庆工商大学催化与功能分子重庆市重点实验室
3. 重庆工商大学环境与生物工程学院

基金项目:

重庆工商大学科学研究项目(1352010)；

详细信息

作者简介:
谭明亮 (1990-) 男, 硕士研究生, 研究方向:生物质资源化利用。;

陈刚 (1974-) 男, 博士, 副研究员, 研究方向:天然产物抗炎免疫调节作用与机制研究。;

中图分类号: R151
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出版历程
- 收稿日期: 2014-09-26

Study on effect and mechanism of tea polyphenols on serum uric acid in hyperuricemic mice

TAN Ming-liang^1,2,
CHEN Gang^1,2,3

Chongqing Key Laboratory of Nature Medicine Research, Chongqing Technology and Business University
Chongqing Key Laboratory of Catalysis and Functional Organic Molecules, Chongqing Technology and Business University
School of Environmental and Biological Engineering, Chongqing Technology and Business University

摘要

摘要: 为探讨茶多酚对氧嗪酸钾诱导的小鼠高尿酸血症的影响及机制,以制备氧嗪酸钾诱导的小鼠高尿酸血症模型,连续给予灌胃150、300、600mg/kg茶多酚7d,磷钨酸法检测小鼠血清尿酸含量,化学比色法检测小鼠血清及肝脏中的黄嘌呤氧化酶活性。结果表明:在茶多酚灌胃300、600mg/kg剂量下与模型组相比高尿酸血症模型小鼠血清中尿酸的含量减少22%及29%(均p<0.01),与此同时,血清中黄嘌呤氧化酶的活性显著降低14%(p<0.05)及21%(p<0.01),且肝脏中黄嘌呤氧化酶的活性也显著降低16%(p<0.05)及21%(p<0.01)。因此茶多酚可显著降低高尿酸血症模型小鼠的血尿酸水平,且其作用机制与抑制黄嘌呤氧化酶的活性密切相关。
- 茶多酚 /
- 高尿酸血症 /
- 黄嘌呤氧化酶 /
- 尿酸
Abstract: To explore the effect of tea polyphenols on hyperuricemia (HUA) in mice and its potential mechanism, HUA was induced by intragastric administration of potassium oxonate in mice. HUA mice were treated with150, 300 and 600mg/kg tea polyphenols for 7 days. Uric acid level in serum was tested by phosphotungstic acid assay. Xanthine oxidase activity in serum and liver was determined by chemical colorimetric assay.Results showed that after treatment with 300 and 600mg/kg tea polyphenols, serum level of uric acid markedly reduced by approximate 22% and 29% (both p<0.01) compared with that of model group. Meanwhile, xanthine oxidase activity in serum markedly decreased by approximate 14% (p<0.05) and 21% (p<0.01) , as well as the liver xanthine oxidase activity markedly decreased 16% (p <0.05) and 21% (p <0.01) . In conclusion, tea polyphenols might reduce the level of uric acid in hyperuricemic mice, and the hypouricemic effect of tea polyphenols might be closely associated with the inhibition of xanthine oxidase activity.
- tea polyphenols /
- hyperuricemia /
- xanthine oxidase /
- uric acid

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