Abstract: Ozone and ultraviolet combined on Lycium barbarum L. dry fruit sterilization process. Sterilization process conditions were further optimized using response surface methodology as follow :radiation distance 31 cm, processing time 32 min, the ozone concentration 31mg/m3, for this condition, the sterilizing efficiency of ozone and ultraviolet combined on Lycium barbarum L. could be 99.999%. The quality of Lycium barbarum L. dry fruit did not change significantly after treatment under optimum conditions (p >0.05) . This result indicating that the compound effects of ozone and ultraviolet had a certain advantages in terms of sterilization and provided scientific data and effective technical means for microbes degradation of Lycium barbarum L. Ningxia.