锐孔凝固浴法制备表面活性肽微胶囊

张生生; 闫静芳; 陆兆新; 张丽娟; 武奔月; 陈阳阳; 王昱沣

doi:10.13386/j.issn1002-0306.2015.12.038

锐孔凝固浴法制备表面活性肽微胶囊

南京农业大学食品科技学院

基金项目:

中央高校基本业务专项基金(KYZ201154)；江苏高校优势学科建设工程资助项目和江苏省优势学科人才引进项目(80900210521)；

详细信息

作者简介:
张生生 (1989-) , 男, 在读硕士研究生, 主要从事食品科学和生物发酵方面的研究。;

王昱沣 (1976-) , 男, 副教授, 主要从事生物分离方面的研究。;

中图分类号: TS201.2
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出版历程
- 收稿日期: 2014-09-23

The preparation of surfactin microcapsules by the method of piercing-solidifying

College of Food Science and Technology, Nanjing Agriculture University

摘要

摘要: 采用锐孔凝固浴法制备表面活性肽微胶囊,并以海藻酸钠为壁材,氯化钙溶液作为固化液。在单因素的基础上,以包埋率作为评价指标,通过响应面实验对微胶囊制备过程中的氯化钙浓度、芯壁比、海藻酸钠浓度、操作温度四个因素进行优化。结果表明:氯化钙浓度为2%,操作温度为49℃,海藻酸钠与表面活性肽比例为1∶2,海藻酸钠浓度为2.4%时,包埋率达到了87.6%,载药量为12.5%。通过肠液缓释实验发现,微胶囊在9h内的释放率达到了91.7%,具有很好的缓释效果。
- 锐孔凝固浴法 /
- 微胶囊 /
- 表面活性肽 /
- 响应曲面法
Abstract: In this paper, piercing-solidifying method was applied to obtain the capsules of surfactin with sodium alginate as wall material and calcium chloride as solid solvent. On the basement of one-factor experiments, four factors including calcium chloride and sodium alginate concentration, ratio of core to wall, temperature were optimized by the method of response surface. The performance of all factors was evaluated by the microencapsulation efficiency. The results showed, under the condition of calcium chloride concentration 2%, temperature 49℃, the proportion of sodium alginate to surfactin 1 ∶2, sodium alginate concentration 2.4%, the microencapsulation efficiency reaches 87.6%, the load ratio of drug reaches 12.5%. The release rate of Surfactin from the microcapsule reached 91.7%, which showed that it had good slow-release effect.
- piercing-solidifying method /
- microcapsules /
- surfactin /
- response surface method

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参考文献(14)

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