新疆酸驼乳NSLAB的筛选及鉴定

樊哲新; 李宝坤; 李开雄; 朱敏; 蒋琰洁; 李王强; 宁孔卵

doi:10.13386/j.issn1002-0306.2015.12.027

新疆酸驼乳NSLAB的筛选及鉴定

石河子大学食品学院

基金项目:

国家自然基金青年项目(31201395);国家自然基金地区项目(31460007);国家自然基金地区项目(30960001)新疆生产建设兵团博士资金专项(2014BB005)；石河子大学高层次人才启动项目(RCZX201223)；

详细信息

作者简介:
樊哲新 (1987-) , 女, 在读硕士研究生, 研究方向:畜产品加工与安全。;

李宝坤 (1979-) , 男, 博士, 副教授, 研究方向:畜产品加工与质量安全。;

李开雄 (1956-) , 男, 教授, 研究方向:畜产品加工与质量安全。;

中图分类号: TS252.1
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出版历程
- 收稿日期: 2014-09-14

Screening and identification of NSLAB in Xinjiang acid camel milk

College of Food Science and Technology, Shihezi University

摘要

摘要: 以新疆牧民家庭自然发酵酸驼乳为原料,采用改良的ROGOSA培养基筛选出20株非发酵剂乳酸菌（Non Starter Lactic Acid Bacteria,NSLAB）,以产酸、自溶度、氨肽酶活力、蛋白水解能力为指标复筛性能优良的NSLAB,为新疆特色干酪的现代化改造提供新的菌株资源。结果表明:从酸驼乳中复筛得到六株NSLAB（编号X-4、X-5、X-7、X-12、X-13、X-15）。据多元统计分析六株菌综合得分X-5>X-4>X-15>X-13>X-12>X-7。其中X-5Δp H（24h）、自溶度、氨肽酶活力、蛋白水解能力分别为1.08%、28.26%、18.88、3.21U/m L,有较好的应用前景。基于菌株生理生化特性,结合16S r DNA基因序列分析,构建菌株系统发育树明确其分类地位,X-4、X-5、X-13、X-15鉴定为植物乳杆菌（Lactobacillus plantarum）,X-7鉴定为发酵乳杆菌（Lactobacillus fermentum）,X-12确定为乳酸片球菌（Pediococcus acidilactici）。
- 发酵乳制品 /
- 非发酵剂乳酸菌 /
- 酸驼乳 /
- 菌种筛选
Abstract: A total of 20 strains Non Starter Lactic Acid Bacteria (NSLAB) were isolated from home-made fermented camel milk samples of Xinjiang through improved ROGOSA medium. Comprehensive 4 physiological indices including the ability of acidity and the proteolysis, extent of autolysis, the aminopeptidase activity, of NSLAB were used to evaluate the composite score and select evaluation indices in tested high properties NSLAB. By multivariate statistical analysis and composite scores (X-5>X-4>X-15>X-13>X-12>X-7) . The results showed that Δp H (24h) , the extent of autolysis, the aminopeptidase activity, the proteolysis ability of X-5 were 1.08%, 28.26%, 18.88, 3.21U/m L, respectively. The isolates were identified by physiology-biochemistry characteristics and the molecular method of 16 S r DNA and a phylogenetic tree was contructed. X-4, X-5, X-13, X-15 were identified as Lactobacillus plantarum, X-7, X-12 were identified as Lactobacillus fermentum, Pediococcus acidilactici, respectively.
- fermented milks /
- Non Starter Lactic Acid Bacteria (NSLAB) /
- fermented camel milk /
- screening

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参考文献(20)

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