Abstract: In order to explore the Fourier transform near- infrared spectroscopy ( FT- NIR) rapid non- destructive detection methods of egg protein, the near- infrared spectroscopy ( 12000⁴000cm- 1) of 83 fresh breeding eggs in equatorial side and sharp side were collected, then the Kjeldahl method was used to determine the crude protein content of correspond eggs, and by First Derivative ( FD) , Second Derivative ( SD) , Multiplication Scatter Correction ( MSC) , Standard Normalized Variate ( SNV) and Spectroscopic Transformation ( ST) of The Unscrambler pretreated spectroscopy. Combined with partial least squares ( PLS) method with leverage correction to establish the mathematical model of the protein, and through the model to determine the feasibility of FT- NIR rapid non-destructive inspection of egg protein. The results showed that: Linear relationship exists between the egg protein and its near- infrared spectroscopy signal, the protein correction model with the equatorial and sharp side original spectrum above 0.74; In the different pretreatment, the model with SD treatment was best effect, equatorial position and sharp- side model R2 were above 0.97, values of RMSEE, RMSEP were small and close to each other; With the treatment of SD, equatorial position and the sharp- side spectrum model had no significant difference, R2, RMSEE, RMSEP were closer.By using FT- NIR rapid non- destructive detection the content of egg protein was feasible.