绿茶多糖的乙酰化修饰及其清除自由基、NO<sub>2</sub><sup>-</sup>活性的研究

梁少茹; 肖霄; 肖斌

doi:10.13386/j.issn1002-0306.2015.11.009

绿茶多糖的乙酰化修饰及其清除自由基、NO₂^-活性的研究

1. 西北农林科技大学园艺学院
2. 北京林业大学林木生物质化学北京市重点实验室

基金项目:

国家茶叶产业技术体系(CARS-23)；陕西省科技统筹工程计划难题招标(2013KTZB02-01-01)；

详细信息

作者简介:
梁少茹 (1987-) , 女, 硕士研究生, 研究方向:茶叶生物化学。;

肖斌 (1957-) , 男, 学士, 教授, 主要从事茶叶生理生态及茶叶生物化学研究。;

中图分类号: TS272.51;TQ281
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出版历程
- 收稿日期: 2014-09-08

Study on acetylated modification and scavenging effect on free radicals, NO2- of green tea polysaccharides

College of Horticulture, Northwest A&F University
Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University

摘要

摘要: 探讨乙酰化修饰对绿茶多糖清除自由基、NO-2活性的影响,设计正交实验研究乙酰化修饰的最佳反应条件,在此条件下制取乙酰化绿茶多糖,对其修饰前后超氧阴离子（O-2·）自由基、羟自由基（·OH）和NO-2体外清除活性进行比较,并进行不同取代度的乙酰化茶多糖进行自由基及NO-2的清除作用实验,考察清除活性与取代度之间的关系。实验所得到乙酰化修饰最优条件为:茶多糖质量（g）与乙酸酐体积（m L）比为1∶40,保温时间4h,反应温度为30℃,乙酰化绿茶多糖最大取代度为0.337,乙酰化绿茶多糖对O-2·、·OH和NO-2体外清除活性最大提高率分别为32.06%、36.96%,55.99%,随着取代度的增大,O-2·、·OH及NO-2的清除活性均增大。研究证明乙酰化修饰能够提高绿茶多糖自由基、NO-2清除活性,且取代度与清除活性有关。
- 绿茶多糖 /
- 乙酰化 /
- 自由基 /
- 亚硝基 /
- 清除活性
Abstract: The experiments was conducted to study the influence of acetylated modification on scavenging free radicals, NO-2effect of green tea polysaccharides. Orthogonal experiment was designed to study the optimal conditions of acetylated modification. Acetylated green tea polysaccharides was produced on this optimal conditions, and superoxide anion free radical, hydroxyl free radical and NO-2scavenging effect in vitro were compared before and after modification.Superoxide anion free radical, hydroxyl radical and NO-2scavenging effect experiments with different substitution degree acetylated tea polysaccharide were conducted, and relationship between the different substitution degree and scavenging activities were analyzed.The optimal conditions of acetylated modification were: mass of tea polysaccharide / volume of acetic anhydride 1 ∶ 40, holding time 4h, reaction temperature 30℃, the maximum substitution degree was 0.337 in this experiment, and the maximum increased ratios of superoxide anion free radical, hydroxyl free radical and NO-2scavenging effects were 32.06%, 36.96%, 55.99%. Besides, superoxide anion free radical, hydroxyl radical and NO-2scavenging activities were increased with the increase of substitution degree. The results showed that acetylated modification could improve the effect of free radicals, NO-2scavenging activities of green tea polysaccharides and scavenging activities was related with substitution degree.
- green tea polysaccharides /
- acetylation /
- free radicals /
- NO-2 /
- scavenging effect

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