Study on acetylated modification and scavenging effect on free radicals, NO2- of green tea polysaccharides
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摘要: 探讨乙酰化修饰对绿茶多糖清除自由基、NO-2活性的影响,设计正交实验研究乙酰化修饰的最佳反应条件,在此条件下制取乙酰化绿茶多糖,对其修饰前后超氧阴离子(O-2·)自由基、羟自由基(·OH)和NO-2体外清除活性进行比较,并进行不同取代度的乙酰化茶多糖进行自由基及NO-2的清除作用实验,考察清除活性与取代度之间的关系。实验所得到乙酰化修饰最优条件为:茶多糖质量(g)与乙酸酐体积(m L)比为1∶40,保温时间4h,反应温度为30℃,乙酰化绿茶多糖最大取代度为0.337,乙酰化绿茶多糖对O-2·、·OH和NO-2体外清除活性最大提高率分别为32.06%、36.96%,55.99%,随着取代度的增大,O-2·、·OH及NO-2的清除活性均增大。研究证明乙酰化修饰能够提高绿茶多糖自由基、NO-2清除活性,且取代度与清除活性有关。Abstract: The experiments was conducted to study the influence of acetylated modification on scavenging free radicals, NO-2effect of green tea polysaccharides. Orthogonal experiment was designed to study the optimal conditions of acetylated modification. Acetylated green tea polysaccharides was produced on this optimal conditions, and superoxide anion free radical, hydroxyl free radical and NO-2scavenging effect in vitro were compared before and after modification.Superoxide anion free radical, hydroxyl radical and NO-2scavenging effect experiments with different substitution degree acetylated tea polysaccharide were conducted, and relationship between the different substitution degree and scavenging activities were analyzed.The optimal conditions of acetylated modification were: mass of tea polysaccharide / volume of acetic anhydride 1 ∶ 40, holding time 4h, reaction temperature 30℃, the maximum substitution degree was 0.337 in this experiment, and the maximum increased ratios of superoxide anion free radical, hydroxyl free radical and NO-2scavenging effects were 32.06%, 36.96%, 55.99%. Besides, superoxide anion free radical, hydroxyl radical and NO-2scavenging activities were increased with the increase of substitution degree. The results showed that acetylated modification could improve the effect of free radicals, NO-2scavenging activities of green tea polysaccharides and scavenging activities was related with substitution degree.
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Keywords:
- green tea polysaccharides /
- acetylation /
- free radicals /
- NO-2 /
- scavenging effect
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