响应面分析法优化超声提取兰坪被毛孢多糖和D-甘露醇

杜亚茜; 虞泓; 葛锋; 刘迪秋; 陈朝银

doi:10.13386/j.issn1002-0306.2015.08.053

响应面分析法优化超声提取兰坪被毛孢多糖和D-甘露醇

1. 昆明理工大学,生命科学与技术学院
2. 云南大学中草药生物资源研究所云百草实验室

基金项目:

国家自然科学基金项目(31260070)；

详细信息

作者简介:
杜亚茜(1989-),女,硕士研究生,研究方向:应用真菌学。;

葛锋(1979-),男,博士,教授,研究方向:植物代谢工程,生物制药。;

中图分类号: TQ461
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出版历程
- 收稿日期: 2014-08-13

Optimization of ultrasonic extraction of polysaccharides and D-mannitol from Hirsutella lanpingensis by response surface methodology

Faculty of Life Science and Technology,Kunming University of Science and Technology
Yunnan Herbal Laboratory,Institute of Herb Biotic Resources,Yunnan University

摘要

摘要: 以兰坪虫草无性型（兰坪被毛孢）固体发酵产物为材料,以水为提取溶剂,在常温条件下利用响应面分析法优化虫草多糖和D-甘露醇的超声提取综合工艺。在超声常温水提的单因素实验的基础上,运用响应面法进一步考察液固比、超声时间、超声功率对虫草多糖和D-甘露醇综合提取率的影响,以优化提取工艺。实验得到较优综合提取工艺条件为:液固比43∶1m L/g,超声时间为50min,超声功率为56W。在该工艺条件下虫草多糖提取率为23.06%,D-甘露醇提取率为2.30%。
- 兰坪虫草 /
- 兰坪被毛孢 /
- 响应面法 /
- 超声提取
Abstract: The ultrasonic extraction technology of polysaccharides and D-mannitol was optimized by response surface methodology at room temperature. The polysaccharides and D-mannitol were extracted by water from the solid fermentation culture of Ophiocordyceps lanpingensis anamorph(Hirsutella lanpingensis). On the basis of single factor ultrasonic extraction in water at room temperature,the effects of ultrasonic time,ratio of liquid to material and ultrasonic power were investigated to extract polysaccharides and D-mannitol,and the technology was optimized by response surface methodology. The results showed that the optimum technology was as follows:the liquid-solid ratio 43∶1m L/g,extraction time 50 min,and extraction power 56 W. Under the conditions,the extraction rates of polysaccharides and D-mannitol were 23.06% and 2.30%,respectively.
- Ophiocordyceps lanpingensis /
- Hirsutella lanpingensis /
- response surface methodology /
- ultrasonic extraction

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