TritonX-100-硫酸铵双水相体系分离地榆根总黄酮
Optimization of Triton X-100-(NH4)_2SO4 aqueous two-phase extraction of total flavonoids from Sanguisorba officinalis L. root
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摘要: 目的:研究利用双水相体系萃取地榆根总黄酮提取的最佳工艺条件。方法:探讨了Triton X-100质量分数,硫酸铵质量分数,黄酮粗提液质量分数、Na Cl质量分数和p H等因素对总黄酮萃取率的影响,并采用Box-Benhnken实验确定了最佳实验条件。结果:双水相萃取黄酮最佳条件为,确定双水相体系组成15%Triton X-100/15%硫酸铵,黄酮粗提液质量分数12%、Na Cl质量分数0.5%、p H6,地榆根总黄酮前萃取率达到80.02%;反萃取的最佳条件:取1.5m L黄酮萃取上相和2m L乙酸丁酯混合,反萃取率为80.03%。结论:采用双水相萃取地榆根总黄酮简单、易行,生产成本低,在中药提取中具有良好应用前景。Abstract: Objective:To study the extracting process of total flavonoids in the roots of Sanguisorba officinalis L.by using aqueous two-phase system. Methods :The effects of Triton X-100 concentration,ammonium sulfate concentration,crude extract concentration,Na Cl concentration and p H value on the extraction efficiency of total flavonoids were explored by one-factor-at-a-time and Box-Benhnken design methods. Results:The optimal aqueous two-phase extraction conditions were 15% Triton X-100,15% ammonium sulfate,crude extract mass concentration 12%,Na Cl concentration 0.5% and p H6. The forward extraction yields of total flavonoids reached80.02%. The optimal condition of backward extraction were as follows :butyl acetate 2m L,the upper phase1.5m L. The efficiency of backward extraction was 80.03%. Conclusion:This method was not only simple but also low-cost. Therefore it would have a well application perspective.
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