Abstract: To established the gel chromatography-gas chromatography(GPC-GC-FID) method for the assay of squalene in several edible oils of plant and animal,the oils were extracted by cyclohexane-ethyl acetate(1∶1,V / V), then purified by GPC to remove macromolecule grease, and then squalene was determined by gas chromatography. Under the experimental condition,squalene showed a good linear relationship in the range of9.40~940μg·m L-1,R2 was 0.9999. The recovery was 95.7%,RSD was 3.0%. The method had the advantage in small sample matrix interference,high sensitivity,avoiding contamination instrument,could be widely used in the analysis of squalene in edible oils of plant and animal sample,which provided the basis for judging criteria of edible oils of plant and animal sample.