响应面法优化牛膝中齐墩果酸超声波辅助提取工艺

赵明霞; 欧阳杰

doi:10.13386/j.issn1002-0306.2015.01.048

响应面法优化牛膝中齐墩果酸超声波辅助提取工艺

赵明霞^1,2,
欧阳杰^1,2

1. 北京林业大学生物科学与技术学院食品科学与工程系
2. 林业食品加工与安全北京市重点实验室(北京林业大学)

基金项目:

第44批回国留学人员科研启动基金；

详细信息

作者简介:
赵明霞(1988-),女,硕士研究生,研究方向:食品加工与安全。;

欧阳杰(1971-),男,博士,副教授,研究方向:食品生物技术。;

中图分类号: TS218
计量
- 文章访问数: 0120
- HTML全文浏览量: 023
- PDF下载量: 0205
出版历程
- 收稿日期: 2014-04-14

Optimization of ultrasonic assisted extraction of oleanolic acid from achyranthes by response surface methodology

ZHAO Ming- xia^1,2,
OUYANG Jie^1,2

Department of Food Science and Engineering,College of Biological Science and Technology,Beijing Forestry University
Beijing Key Laboratory of Forest Food Processing and Safety,Beijing Forestry University

摘要

摘要: 以牛膝为原料,以齐墩果酸提取得率为指标,采用超声波辅助提取牛膝中齐墩果酸。分别研究了乙醇浓度、料液比、提取时间对齐墩果酸提取得率的影响,通过响应面实验优化了提取工艺。结果表明,齐墩果酸提取的较佳工艺条件是:乙醇浓度70%,提取时间20min,料液比(w/v)1∶20。在此条件下,齐墩果酸的提取含量为11.14mg/g。
- 牛膝 /
- 齐墩果酸 /
- 超声波辅助 /
- 提取工艺
Abstract: Oleanolic acid was extracted from achyranthes root by ultrasonic assisted extraction. The effects of ethanol concentration,solid- liquid ratio,and extraction time on the extraction yield of oleanolic acid were investigated.The extraction technology was optimized by response surface methodology. The results showed that the optimal extraction conditions of oleanolic acid were as follows: ethanol concentration was 70%,extraction time was 20 min,and solid- liquid ratio was 1 ∶20. Under above conditions,the extraction yield of oleanolic acid reached11.14 mg / g.
- Achyranthes /
- oleanolic acid /
- ultrasound assisted /
- extraction

HTML全文

参考文献(14)

[1]	王广树,丛登立,杨锦竹,等.牛膝中三萜皂苷的研究[J].中国药物化学杂志,2005,15(4):224-226.
[2]	CL de Melo,MGR Queiroz,et al.Oleanolic acid,a natural triterpenoid improves blood glucose tolerance in normal mice and ameliorates visceral obesity in mice fed a high-fat diet[J].Chemico-Biological Interactions,2010,185(1):59-65.
[3]	王汉屏,王立志.木瓜齐墩果酸的研究进展[J].食品科学,2007,28(10):621-623.
[4]	Z Wang,C Hsu,C Huang,et al.Anti-glycative effects of oleanolic acid and ursolic acid in kidney of diabetic mice[J].European Journal of Pharmacology,2010,628(1):255-260.
[5]	盛侠.宣木瓜中齐墩果酸的提取分离及其抗氧化作用的研究[D].合肥:安徽农业大学,2010.
[6]	KI Wolska,AM Grudniak,B Fiecek,et al.Antibacterial activity of oleanolic and ursolic acids and their derivatives[J].Central European Journal of Biology,2010,5(5):543-553.
[7]	汤华成,张东杰,赵蕾.功能性食品因子齐墩果酸研究进展[J].食品科技,2007,9:16-18.
[8]	J Pollier,A Goossens.Oleanolic acid[J].Phytochemistry,2012,77:10-15.
[9]	KI Wolska,AM Grudniak,B Fiecek,et al.Antibacterial activity of oleanolic and ursolic acids and their derivatives[J].Central European Journal of Biology,2010,5(5):543-553.
[10]	孟大利,李铣.中药牛膝化学成分和药理活性的研究进展[J].中国药物化学杂志,2001,11(2):120-124.
[11]	陶亮亮,马雄,刘柯彤,等.超声波辅助提取枇杷叶中齐墩果酸工艺研究[J].化工技术与开发,2011,40(2):17-20.
[12]	黄志芳,黄志红,胡吉林,等.土牛膝中齐墩果酸、牛膝多糖的提取与鉴定[J].中国实用医药,2010,5(17):6-7.
[13]	赵培西,王四旺,王剑波.牛膝中齐墩果酸提取工艺研究[J].陕西中医,2005,26(10):1099-1101.
[14]	蒋珍菊,王周玉.不同方法提取女贞子中齐墩果酸的含量[J].安徽农业科学,2011,39(26):15979-15981.