Abstract: To study the ultrasonic-microwave synergistic extraction technology of Qaidam Basin Nitraria tangutorum Bobr. oil. The type of extractant, microwave power, ultrasonic power, solvent to solid sample ratio and extraction time were investigated by using single factor experiment method with extraction yield of Nitraria tangutorum Bobr. oil as evaluation index. On the basis of single factor experiment, the factors showed significant effects were selected in the further orthogonal experiments designed by Design-Expert software. The best combination of extraction parameters was obtained with response surface methodology as follows:microwave power 490 W, ultrasonic power 290 W, solvent to sample ratio 20 ∶ 1m L / g and extraction time 8min. Under these best conditions, the extraction yield of Nitraria tangutorum Bobr. oil was 5.71% ±0.18% (n =3) . The results from experimental value are quite consistent with the theoretical expectation value 5.91%, thus regression model could well predict extraction yield of Nitraria tangutorum Bobr. oil. Results of the trial indicated that the ultrasonic-microwave synergistic extraction of Nitraria tangutorum Bobr. oil had many advantages such as high yield, high speed, simple operation and so on. Additionally, the results provided the scientific reference for further study of Nitraria tangutorum Bobr.