蚕蛹蛋白肠内营养制剂干预2型糖尿病小鼠代谢特性的研究

赵清波; 吴文惠; 周喻; 朱长林; 陈佳捷; 张朝燕; 包斌

doi:10.13386/j.issn1002-0306.2014.23.064

蚕蛹蛋白肠内营养制剂干预2型糖尿病小鼠代谢特性的研究

1. 上海海洋大学食品学院
2. 上海海洋大学海洋药物与健康食品研究中心
3. 上海市第六人民医院

基金项目:

上海市科委工程中心建设项目(11DZ2280300)；上海海洋大学科技发展专项基金；

详细信息

作者简介:
赵清波 (1987-) , 女, 硕士研究生, 研究方向:食品工程专业。;

包斌 (1967-) , 女, 硕士, 研究方向:食品科学与工程。;

中图分类号: R587.1;TS201.4
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出版历程
- 收稿日期: 2014-03-21

Silkworm pupa protein-based enteral formula for improving metabolic characteristic in streptozotocin-induced Type 2 Diabetic mice

College of Food Science and Technology, Shanghai Ocean University
Institute of Marine Drug and Healthy Food, Shanghai Ocean University
Shanghai Sixth People's Hospital

摘要

摘要: 目的:研究蚕蛹短肽肠内营养(EN)制剂和蚕蛹蛋白EN制剂对高脂饲料和链脲佐菌素诱导的2型糖尿病(T2DM)小鼠代谢特性的影响。方法:糖尿病小鼠分为5组(n=10只),分别是空白组、益力佳组、蚕蛹短肽1组、蚕蛹短肽2组及蚕蛹蛋白组,并随机选取10只正常小鼠作为对照组。各组小鼠采用对应EN制剂支持四周并测定其空腹血糖值(FBG)、口服葡萄糖耐量及血清生化指标。结果:DM小鼠在接受以蚕蛹短肽或蚕蛹蛋白为蛋白源的EN制剂支持两周后其FBG值均能显著降低(p<0.01),相对于空白组,短肽1组、短肽2组和蚕蛹蛋白组的FBG水平分别降低了47.47%、38.14%和41.52%。同时,以蚕蛹短肽为蛋白源的EN制剂能显著改善糖尿病小鼠的葡萄糖耐量(p<0.01);蚕蛹短肽或蚕蛹蛋白为蛋白源EN制剂还能明显降低糖尿病小鼠的总胆固醇(TC)(p<0.01)、总甘油三脂(TG)及低密度脂蛋白(LDL)含量。结论:以蚕蛹短肽或蚕蛹蛋白为蛋白质来源的特殊医学用途配方食品能显著降低2型糖尿病小鼠血糖值,并有一定的调节血脂代谢的作用。
- 蚕蛹蛋白 /
- 糖尿病 /
- 肠内营养 /
- 肠内营养支持制剂
Abstract: Objective: The present study was designed to investigate the silkworm pupa protein or peptide- based enteral formula for improving metabolic characteristic in streptozotocin- induced type 2 diabetes mellitus ( T2DM) mice.Method: T2 DM mice model was randomly assigned to 5 groups ( n = 10 / group) .The 1stgroup, blank group fed with Standard chow, the 2ndand 3rdgroup fed with Abbott Glucerna SR and silkworm pupae peptide sample1- based EN ( spp1) , respectively. The 4thand 5thgroup fed with silkworm pupae peptide sample 2- based EN ( spp2) and silkworm pupae protein sample 3-based EN ( spp3) , respectively.The levels of fasting blood glucose ( FBG) , oral glucose tolerance test ( OGTT) and blood biochemical indices were determined. Results: The SPP Enteral Formula could significantly reduce blood glucose in T2 DM mice ( p < 0.01) compared with the control group.FBG of spp1, spp2 and spp3 was decreased with the value of 47.47%, 38.14% and 41.52%, respectively. The results of OGTT showed that two SPP groups were increased significantly ( p < 0.01) .The level of blood biochemical indices including TC, TG and LDL were decreased in the 3rd and 4th group, which use SPP- based EN. Conclusion: SPP- based Enteral Formula marked decreased the levels of T2 DM mice blood glucose. It also showed the actively effect of improving lipid metabolism.
- Silkworm pupa protein /
- type 2 Diabetes mellitus /
- enteral nutrition /
- enteral nutrition support formula

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