Process optimization of surfactant assisted enzyme extraction of Astragalus flavonoids
-
摘要: 以乙醇为提取剂,采用表面活性剂辅助纤维素酶解法预处理从黄芪中提取黄酮,在单因素实验结果基础上,利用Box-Behnken的中心组合实验设计,运用响应面分析法对影响黄芪黄酮得率的主要因素(溴化十六烷基三甲胺(CTMAB)添加量、提取温度、乙醇浓度)进行优化,得出黄芪黄酮的最佳提取工艺条件为:CTMAB添加量0.37%,乙醇浓度80%,提取温度79℃,在此条件下黄酮得率实测值为4.192%,理论值为4.287%,实测值与理论值相差较小。Abstract: The flavonoids was extracted by surfactant- assisted enzymatic pretreatment with alcohol as the solvent from Astragalus.Experiment factors and levels were firstly selected by one- factor tests. According to the Box-Benhnken center- united experimental design principles, the method of response surface analysis was adopted to optimize extraction of flavonoids.The results showed that the optimum conditions were: addition of CTMAB 0.37%, concentration of ethanol 80%, temperature 79℃.Yield of that optimized procedure was 4.192%, which was close to the predicted value of 4.287%.
-
Keywords:
- Astragalus /
- flavonoids /
- surfactant /
- cellulase /
- extraction
-
[1] 中华人民共和国药典委员会.中国药典 (一部) [S].北京:化学工业出版社, 2005:212-213. [2] 段亚丽, 谢梅冬.黄芪化学成分及其有效成分黄芪甲苷含量测定的研究现状[J].中国兽药杂志, 2005, 39 (3) :35-38. [3] Heim KE, Tagliaferro AR, Bobily D.Flavonoid antioxidants:chemistry, metabolism and structure-activity relationships[J].The Journal of Nutritional Biochemistry, 2002, 13 (10) :572-584.
[4] Wang Deqing, Zhuang Yuan, Tian Yaping, et al.Study of the effects of total flavonoids of Astragalus on atherosclerosis formation and potential mechanisms[J].Oxidative medicine and cellular longevity, 2012, 11 (10) :282-283.
[5] 常美玲, 薛彦朝, 李奉勤, 等.超声波法提取黄芪中黄酮的最佳工艺研究[J].河北医药, 2006 (5) :426-427. [6] 肖卫华, 韩鲁佳.黄芪黄酮乙醇回流提取工艺的研究[J].食品工业科技, 2008, 29 (1) :233-235. [7] 贲永光, 丘泰球, 李坤平, 等.纤维素酶法提取黄芪总黄酮的工艺研究[J].时珍国医国药, 2009, 20 (10) :2478-2480. [8] 武艳梅, 赵航, 李淑燕, 等.闪式提取法提取黄芪活性成分工艺研究[J].食品科学, 2011, 24:98-101. [9] 李秀信, 张军华.表面活性剂-微波辅助提取香椿黄酮[J].食品与发酵工业, 2011, 37 (1) :199-201. [10] 赵二劳, 范建凤, 张小燕.表面活性剂-尼纳尔增效微波提取沙棘叶黄酮的研究[J].日用化学工业, 2009, 39 (1) :36-40. [11] 曾晶, 叶媛, 龚大春.等.表面活性剂对纤维素酶水解过程的影响[J].酿酒科技, 2008, 12 (1) :38-40. [12] 邓一民.表面活性剂与染料对纤维素酶活性的影响[J].广西纺织科技, 2009, 39 (4) :23-26. [13] 王军, 王敏, 季璐.苦荞麦麸皮总黄酮提取工艺及其数学模型研究[J].农业工程学报, 2006 (7) :223-225. [14] 费荣昌.实验设计与数据处理[M].无锡:江南大学出版社, 2001:59-63. [15] 王倩, 常丽新, 贾长虹.超声波法提取狗枣猕猴桃叶黄酮的工艺研究[J].食品研究与开发, 2012, 33 (2) :43-46.
计量
- 文章访问数: 161
- HTML全文浏览量: 29
- PDF下载量: 334