甜菜渣粗多糖的酶法提取工艺、组成及抑菌活性研究

李云姝; 李博生

doi:10.13386/j.issn1002-0306.2014.23.018

甜菜渣粗多糖的酶法提取工艺、组成及抑菌活性研究

李云姝¹,
李博生²

1. 锡林郭勒职业学院
2. 北京林业大学

详细信息

作者简介:
李云姝 (1970-) , 女, 硕士, 副教授, 研究方向:生物学。;

中图分类号: TS249;TQ281
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- 文章访问数: 134
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出版历程
- 收稿日期: 2014-03-19

Optimization of cellulase- assisted extraction of sugarbeet boot pomace crude polysaccharides, compositions and antibacterial activity

LI Yun-shu¹,
LI Bo-sheng²

Xilin Gol Vocational College
Beijing Forestry University

摘要

摘要: 为研究不同提取条件对甜菜渣粗多糖(SCPs)的影响,以便优化SCPs的提取工艺条件。在单因素实验的基础上,进行4因素3水平的响应面分析,优化纤维素酶辅助提取SCPs的条件;同时研究了SCPs的理化成分、单糖组成和抑菌活性。结果表明,最佳提取工艺为料液比(蒸馏水体积∶甜菜质量)30∶1、酶解时间40min、酶解温度52℃、p H4.5、酶用量4677U/g,在此条件下SCPs得率为3.93%。抑菌实验显示:甜菜渣粗多糖对金黄色葡萄球菌、枯草芽孢杆菌、大肠杆菌均有抑制作用,其最小抑菌质量浓度分别为12.5、25、25mg/m L。
- 甜菜渣 /
- 粗多糖 /
- 响应面 /
- 组成 /
- 抑菌活性
Abstract: In order to study the different parameter of crude polysaccharides from sugarbeet boot pomace, the best extracting process was ensured.Based on one- factor- at- a- time experiments, response surface methodology with four- factors- three- levels was used to optimize the cellulase- assisted extraction of crude polysaccharides from sugarbeet boot pomace ( SCPs) . The physicalchemical compositions, monosaccharide compositions and antibacterial activity of SCPs were investigated. The optimal extraction conditions was found as 30 ∶ 1 of distilled water-to-sugarbeet boot, hydrolysis time 40 min, hydrolysis temperature 52℃, p H4.5, and cellulase amount 4677 U / g.Under these conditions, the extraction yield of SCPs was 3.93%. The antibacterial assay showed that these polysaccharides had different antibacterial activity against Staphyloccocus aureus, Bacillus subtilis and Escherichia coli, and the corresponding minimum inhibitory concentrations ( MICs) were 12.5, 25 and 25 mg / m L, respectively.
- sugarbeet boot pomace /
- crude polysaccharides /
- response surface methodology /
- compositions /
- antioxidant activities

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参考文献(21)

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