Abstract: An appropriate enzymatic system was preliminary explored for the Yarrowia lipolytica lipase. The study found that the optimal enzyme reaction system for the whole lipase powder was 50mmol/L p H7.0 Tris-HCl (containing 7.5mmol/L ammonium taurocholate) , 100mmol/L p H6.0 PBS (containing 1000 U lipase powder) , and the ferulic acid yield was 2.94%. To further improve the efficiency of hydrolysis of lipase, lip2 lipase gene was cloned and integrated in Pichia pastoris GS115 genome, and the lip2 lipase powder was obtained by fermentation. Using the optimal enzyme reaction system to hydrolysis oryzanol, the results showed that the ferulic acid yield by lip2 lipase powder was 2.87%. This efficiency was slightly lower than the whole lipase powder hydrolysis efficiency. However, a single lipase gene was obtained, which would lay the foundation of improving the catalyze ability to hydrolysis oryzanol by molecular evolution technology.