Effect of extract of pine needle on colonic antioxidant capacity and fecal excretion in chronic constipated mice
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摘要: 探讨松针提取液对慢性便秘小鼠通便功能及肠道抗氧化能力的影响。将76只雄性小鼠按体重随机分在空白组、便秘组、1∶1、1∶20和1∶40（g∶m L）松针组、1∶20（g∶m L）大黄组。复方地芬诺酯造模后,给予各剂量组相应提取液干预（1次/d）,给予便秘组和空白组生理盐水。一周后评价松针提取液的通便作用和肠道的抗氧化能力。结果显示,造模两周后,便秘组丙二醛（MDA）含量比空白组显著升高（p<0.01）。经松针提取液干预后,小鼠排便时间缩短（p<0.05）,排便的粒数和重量增加（p<0.05）,MDA含量降低（p<0.05）,超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）活性增强（p<0.05）,一氧化氮（NO）的含量减少（p<0.05）。说明松针提取液能提高肠道抗氧化能力和促进通便的作用。Abstract: The effect of extract of pine needle on colonic antioxidant capacity and fecal excretion of chronic constipated mice was investigated.Seventy- six mice were randomly divided into control group, constipated group, three dose groups ( 1 ∶ 1, 1 ∶ 20, 1 ∶ 40 g ∶ m L pine needle) and positive group ( 1 ∶ 20 g ∶ m L rhubarb) . The constipated group was induced by lomotil solution ( 5mg / kg BW) once a day for two weeks. Three dose groups were given intervention with relevant extract of pine needle and others were given saline. One week later, defecation function and colonic antioxidant capacity was tested. To data, MDA was remarkably increased in colon after two weeks lomotil treatment.After the intervention of pine needle, the laxative activity of the mice was enhanced ( p < 0.05) ;SOD, GSH- Px activity was improved and MDA, NO content was decreased ( p < 0.05) . The results lead to the conclusion that the pine needle extract can improve colonic antioxidant capacity of constipated mice and relieve constipation.
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Keywords:
- pine needle /
- antioxidant capacity /
- laxative activity /
- mice
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