Study on ultrasonic wave extraction and antioxidant of polysaccharides from Piptoporus betulinus Karst
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摘要: 目的:优化桦黄多糖的超声提取工艺条件并初步探讨其抗氧化性能。方法:在单因素实验基础上,以桦黄多糖提取率为评价指标,通过响应面分析方法确定桦黄多糖最佳超声提取工艺条件;通过清除超氧阴离子自由基和DPPH自由基的实验来检测多糖抗氧化性能。结果:桦黄多糖最佳超声提取工艺条件为:温度为62℃,超声功率为220W,时间为35min,液料比为16∶1(mL/g),提取2次,在此优化条件下多糖的平均提取率为7.87%,得到的桦黄多糖具有较好的抗氧化性能。结论:首次得到了一条适用于桦黄多糖超声提取的工艺路线,与传统工艺比较,超声提取法具有提取快、提取率高的优点。Abstract: Objective:To select the optimum extracting with the assistance of ultrasonic method of polysaccharides from Piptoporus betulinus Karst. Methods:The response surface analysis was employed to determine the optimum technological condition. Results : The optimum extraction conditions were as follows : extraction temperature 62℃, ultrasonic power 220 W, extraction time 35 min, ratio of water to raw material 16∶1mL/g and 2times. The yield of polysaccharides was 7.87% based on the above conditions. Conclusion:This method was suitable for the ultrasonic extraction and determination of polysaccharides from Piptoporus betulinus Karst.Compared with the traditional methods, ultrasonic extraction method was time-saving and efficiency.
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[1] 赵继鼎, 徐连旺, 张小青.中国多孔菌科分类系统的研究, [J].微生物学报, 1982, 22 (3) :218-232. [2] 乔蓉霞, 张国跃, 郭耀武, 等.桦黄质量标准研究[J].中国医药导刊, 2011, 13 (8) :1450-1451. [3] 张国跃, 乔蓉霞, 郭耀武, 等.反相HPLC法测定陕西民间习用药材桦黄中麦角甾醇的含量[J].安徽医药, 2011, 15 (11) :1360-1362. [5] 贺婷, 向莹, 陈健.滑子菇多糖超声辅助提取工艺及抗氧化活性评价[J].食品工业科技, 2013, 34 (7) :235-239. [6] 高擎, 游丽君, 赵谋明.超声辅助提取松茸抗氧化多糖工艺的研究[J].食品工业科技, 2012, 33 (7) :298-302. [7] 赵鹏.款冬花多糖提取纯化工艺研究及结构鉴定[D].西安:西北大学, 2010:99. [8] 宋逍, 赵鹏, 申婉容, 等.款冬花硒多糖的制备及抗氧化性研究[J].食品工业科技, 2013, 34 (13) :227-231.
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